复合诱变被孢霉原生质体获高产γ-亚麻酸的研究

以拉曼被孢霉(Mortierella ramanniana)为出发菌株M5, 通过原生质体制备, 经亚硝酸和激光等进行复合诱变; 进行初筛, 经摇瓶发酵法复筛, 并测定其相关性能指标,获得一生产性能比出发菌株显著提高的突变株MF10; 其干菌体收率为46.8 g/L、油脂产率达34.6 g/L、γ-亚麻酸的产率达12.5 g/L, 分别是出发菌株的1.73倍、2.02倍和2.6倍。通过基因传代实验, 说明突变株的基因可稳定遗传。

Abstract

A mutant strain MF10 accumulating a large amount of lipid was successfully obtained when the original strain Mortierella ramanniana-M5 was treated by compound mutagenesis through the use of HNO2 and laser after its cell walls was decomposed. The content of lipid in MF10 mycelium culture in shaking flask with its all dry cell weight was up to 46.8 g/L which is 173% higher than that in the original strain,and its lipid yield 34.5 g/L and γ-Linolenic acid yield 12.5 g/L are 202% and 260% higher than its original strain.The passing generation test showed that the heredity character of mutant MF10 was stable.

参考文献

[1] 李艳明, 安家彦, 宋建国. 雅致小克银汉霉发发酵生产γ-亚麻酸［J］.大连轻工业学院学报, 2000, 4: 277-280.

LI Yanming, AN Jiayan, SONG Jianguo. Fermentation production of γ-linolenic acid by Cunningnarnella elegans［J］. Journal of Dalian Institute of Light Industry, 2000, 4: 277-280.

[2] 曾杨, 余龙江, 朱敏, 等.氮饥饿补糖分批培养小克银汉霉产γ-亚麻酸的研究［J］. 生命科学研究, 2004, 8(1): 86-88.

ZENG Yang, YU Longjiang, ZHU Min, et al.Study on fed-batch culture of strain Cunninghamelia sp.to produce Gamma-linolenic acid by nitrogen starvation and glucose feeding［J］. Life Science Research, 2004, 8(1): 86-88.

[3] 梁凇, 张晓昱, 王宏勋. 影响刺孢小克银汉霉菌菌体形态和γ-亚麻酸含量的因素［J］. 华中农业大学学报, 2005, 24(1): 52-54.

LIANG Song, ZHANG Xiaoyu,WANG Hongxun.Factors affecting morphology of cunninghamella echinulata and production of 7-1inolenic acid［J］. Journal of Huazhong Agricultural University, 2005, 24(1): 52-54.

[4] 赵振英, 周连甲.微生物法制取γ-亚麻酸的研究: Ⅰ.γ-亚麻酸［J］.微生物学杂志, 1991, 11(2): 44-46.

ZHAO Zhenying, ZHOU Lianjia.Research on production of γ-linolenic acid by microbe: Ⅰ.γ-linolenic acid［J］.Journal of Microbiology, 1991, 11(2): 44-46.

[5] 黄建忠, 施巧琴.深黄被孢霉高产脂变株的选育及其发酵的研究［J］.微生物学通报, 1998, 25(4): 187-191.

HUANG Jianzhong,SHI Qiaoqin.Studies on the breeding of Mortierella isabellina mutant high producing lipid and its fermentation conditions［J］. Microbiology, 1998, 25(4): 187-191.

[6] 张玲, 李植峰.雅致枝霉高产γ-亚麻酸突变株的选育［J］.生物技术, 2000, 10(6): 22-24.

ZHANG Ling, LI Zhifeng. Breeding of Thamnidium elegans of high γ-linolenic acid［J］. Biotechnology, 2000, 6: 22-24.

[7] 周蓬蓬, 余龙江, 汪建华, 等.微波等离子体溅射诱变选育花生四烯酸高产菌及补料工艺研究［J］.激光生物学报, 2003, 12(1): 59-62.

ZHOU Pengpeng, YU Longjiang, WANG Jianhua, et al.Study on implanted with low energy nitrogen ion beams in breeding of arachidonic acid producer mortierella alpina and technology of feeding batch［J］.Acta Laser Biology Sinica, 2003, 12(1): 59-62.

[8] 单志萍, 姜文修, 孟纾.丝状真菌三孢布拉霉原生质体的制备与再生的研究［J］. 江苏食品与发酵, 1999, 99(4): 11-14.

SHAN Zhiping, JIANG Wenxiu, MENG Shu. Study on the protoplast isolation and regeneraion of blakeslea trispora of filamentous fungi［J］.Jiangsu Food and Fermentation, 1999, 99 (4): 11-14.

[9] 熊本涛, 王卫卫, 孙继民, 等.小克银汉霉原生质体的制备与再生［J］. 西北大学学报, 2004, 4: 439-442.

XIONG Bentao,WANG Weiwei,SUN Jimin, et al.Protoplast formation and regeneration of γ-linlenic acid producing starins of C.echinulata［J］. Journal of Northwest University (Natural Science Edition), 2004, 4: 439-442.

[10] OSAMU HIRUTA. Application of maxblend fermentor for microbial processes［J］ . Journal of Fermentation and Bioengineering, 1997, 83(1): 79-86.

[11] 宗立立, 宋慧, 付岩松, 等.不同培养条件下滑菇菌株酯酶同工酶的研究［J］. 扬州大学学报, 2010, 2: 78-81.

ZONG Lili, SONG Hui, FU Yansong, et al.Study on the esterase isoenzyme in different culture condition of Pholiota nameko［J］. Journal of Yangzhou University(Agricultural and Life Science Edition), 2010, 2: 78-81.

[12] 萧浪涛, 王三根.植物生理学实验技术［M］.中国农业出版社, 2005: 192-196.

XIAO Langtao,WANG Sangen. Plant physiology experiment technology［M］.China Agriculture Press, 2005: 192-196.

孙继民, 王卫卫, 马庆辉, 杨洁, 邹学校. 复合诱变被孢霉原生质体获高产γ-亚麻酸的研究[J]. 激光生物学报, 2011, 20(6): 824. SUN Jimin, WANG Weiwei, MA Qinghui, YANG Jie, ZOU Xuexiao. Studies on Compound Mutagenesis Method of Protoplast of Mortierella Ramanniana Obtaining High γ-Linolenic Acid Yield Strain[J]. Acta Laser Biology Sinica, 2011, 20(6): 824.

复合诱变被孢霉原生质体获高产γ-亚麻酸的研究

关于本站 Cookie 的使用提示

全站搜索

复合诱变被孢霉原生质体获高产γ-亚麻酸的研究

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索