激光与光电子学进展, 2012, 49 (6): 063001, 网络出版: 2012-05-02   

活体糖尿病小鼠中单个白细胞的拉曼光谱分析

Raman Spectroscopic Analysis of Single White Blood Cell of DM Mouse in vivo
作者单位
1 广西科学院生物物理实验室, 广西 南宁 531003
2 广西师范大学物理科学与技术学院, 广西 桂林 541004
3 玉林市第一中等职业技术学校, 广西 玉林 537000
4 广西医科大学附属肿瘤医院, 广西 南宁 531003
摘要
利用拉曼光镊并结合多元统计分析方法无损地对活体糖尿病(DM)小鼠中的白细胞(WBC)进行了研究,分别检测了糖尿病和正常小鼠活体中的白细胞拉曼光谱,利用主成分分析(PCA)建立拉曼光谱诊断多元统计算法模型。结果表明,患糖尿病小鼠和正常小鼠白细胞拉曼光谱差别明显,且实验存在较好的重现性。利用PCA统计分析方法得到诊断特异性和灵敏度达到了98%。在活体糖尿病小鼠白细胞中出现较高的蛋白质的特征峰1302 cm-1,表明活体糖尿病白细胞中的蛋白质浓度比正常状态高。糖尿病小鼠内的白细胞与正常相比,DNA磷酸骨架基团强度和蛋白质酰胺强度升高,表明DNA双螺旋结构、蛋白质主链和氢键体系发生变化,二级构象改变。
Abstract
Raman spectra of normal white blood cell (WBC) and diabetes mellitus (DM) WBC in vivo are studied by laser tweezers combined with multivariate statistic analysis method. Raman spectrum and multivariate statistic model is founded by the principal components analysis (PCA). The result of Raman spectrum suggests that DM WBC and normal WBC could be differentiated with PCA. Diagnostic specificity and sensitivity are nearly 98%. The protein vibration bands at 1302 cm-1 in DM WBC mouse in vivo show that the concentration of protein in DM WBC is higher than normal WBC. The Raman intensities at O-P-O of DNA and amide bands of DM cell are significantly higher than normal cell, which shows that the changes in the comformation of O-P-O and the backbone and hydrogen bonds of protein, as well as the second comformation of protein.
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牛丽媛, 林漫漫, 李雪, 董云亮, 魏坤莲, 匡志鹏, 刘军贤, 姚辉璐. 活体糖尿病小鼠中单个白细胞的拉曼光谱分析[J]. 激光与光电子学进展, 2012, 49(6): 063001. Niu Liyuan, Lin Manman, Li Xue, Dong Yunliang, Wei Kunlian, Kuang Zhipeng, Liu Junxian, Yao Huilu. Raman Spectroscopic Analysis of Single White Blood Cell of DM Mouse in vivo[J]. Laser & Optoelectronics Progress, 2012, 49(6): 063001.

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