水体细菌微生物多波长透射光谱解析模型

胡玉霞; 赵南京; 甘婷婷; 段静波; 喻慧娟; 孟德硕; 刘建国; 刘文清

doi:doi:10.3788/AOS201737.0830001

光学学报, 2017, 37 (8): 0830001, 网络出版: 2018-09-07

水体细菌微生物多波长透射光谱解析模型下载： 856次

An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water

胡玉霞 ^1,2,3赵南京 ^1,3,*甘婷婷 ^1,3段静波 ^1,3喻慧娟 ^1,2,3孟德硕 ^1,3刘建国 ^1,3刘文清 ^1,3

作者单位

¹ 中国科学院安徽光学精密机械研究所环境光学与技术重点实验室, 安徽合肥 230031

² 中国科学技术大学, 安徽合肥 230026

³ 安徽省环境光学监测技术重点实验室, 安徽合肥 230031

图 & 表

图 1. 大肠杆菌的多波长透射光谱

Fig. 1. Multi-wavelength transmission spectrum of Escherichia coli

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图 2. 细菌微生物中总核酸、发色团氨基酸和吡啶二羧酸的吸收系数[13]

Fig. 2. Absorption coefficients of total nucleic acid, chromophoric amino acid, and dipicolinic acid in bacterial microorganism[13]

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图 3. (a)发色团氨基酸和(b)总核酸复折射率的虚部

Fig. 3. Imaginary part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

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图 4. (a)发色团氨基酸和(b)总核酸复折射率实部的变化量

Fig. 4. Variation in real part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

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图 5. 大肠杆菌测量光谱与模拟光谱的比较

Fig. 5. Comparision between measured and calculated optical density spectra of Escherichia coli

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图 6. 单个大肠杆菌模拟光谱与测量光谱的线性拟合关系

Fig. 6. Correlation between calculated spectra and measured spectra of single Escherichia coli cell

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表 1不同波段下测量光谱与模拟光谱的相对误差

Table1. Relative error between measured and calculated optical density spectra in different bands

Wavelength /nm	240-250	250-400	400-750	750-900	240-900
Maximum relative error /%	6.18	3.11	3.12	9.76	9.76
Average relative error /%	2.09	0.99	0.97	3.67	1.62

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表 2250~750 nm波段范围内大肠杆菌透射光谱模型解析参数

Table2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli

Parameter	Calculated value	Literature/experimental value
Volume /μm³	4.6093	1-7^[23]
Average diameter of external structure $\begin{matrix} {D_{1}}_{} \end{matrix}$ /μm	2.0648	1.9189
Particle number $\begin{matrix} {N_{p}}_{} \end{matrix}$ /mL^-1	20497000	20000000
Average diameter of internal structure $\begin{matrix} {D_{2}}_{} \end{matrix}$ /μm	0.0667
Volume fraction of internal structure /%	30

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胡玉霞, 赵南京, 甘婷婷, 段静波, 喻慧娟, 孟德硕, 刘建国, 刘文清. 水体细菌微生物多波长透射光谱解析模型[J]. 光学学报, 2017, 37(8): 0830001. Yuxia Hu, Nanjing Zhao, Tingting Gan, Jingbo Duan, Huijuan Yu, Deshuo Meng, Jianguo Liu, Wenqing Liu. An Analytical Model for Multi-Wavelength Transmittance Spectra of Bacteria in Water[J]. Acta Optica Sinica, 2017, 37(8): 0830001.

水体细菌微生物多波长透射光谱解析模型下载： 856次

图 1. 大肠杆菌的多波长透射光谱

Fig. 1. Multi-wavelength transmission spectrum of Escherichia coli

图 2. 细菌微生物中总核酸、发色团氨基酸和吡啶二羧酸的吸收系数[13]

Fig. 2. Absorption coefficients of total nucleic acid, chromophoric amino acid, and dipicolinic acid in bacterial microorganism[13]

图 3. (a)发色团氨基酸和(b)总核酸复折射率的虚部

Fig. 3. Imaginary part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

图 4. (a)发色团氨基酸和(b)总核酸复折射率实部的变化量

Fig. 4. Variation in real part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

图 5. 大肠杆菌测量光谱与模拟光谱的比较

Fig. 5. Comparision between measured and calculated optical density spectra of Escherichia coli

图 6. 单个大肠杆菌模拟光谱与测量光谱的线性拟合关系

Fig. 6. Correlation between calculated spectra and measured spectra of single Escherichia coli cell

表 1不同波段下测量光谱与模拟光谱的相对误差

Table1. Relative error between measured and calculated optical density spectra in different bands

表 2250~750 nm波段范围内大肠杆菌透射光谱模型解析参数

Table2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli

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图 1. 大肠杆菌的多波长透射光谱

Fig. 1. Multi-wavelength transmission spectrum of Escherichia coli

图 2. 细菌微生物中总核酸、发色团氨基酸和吡啶二羧酸的吸收系数[13]

Fig. 2. Absorption coefficients of total nucleic acid, chromophoric amino acid, and dipicolinic acid in bacterial microorganism[13]

图 3. (a)发色团氨基酸和(b)总核酸复折射率的虚部

Fig. 3. Imaginary part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

图 4. (a)发色团氨基酸和(b)总核酸复折射率实部的变化量

Fig. 4. Variation in real part of complex refractive index of (a) chromophoric amino acid and (b) total nucleic acid

图 5. 大肠杆菌测量光谱与模拟光谱的比较

Fig. 5. Comparision between measured and calculated optical density spectra of Escherichia coli

图 6. 单个大肠杆菌模拟光谱与测量光谱的线性拟合关系

Fig. 6. Correlation between calculated spectra and measured spectra of single Escherichia coli cell

表 1不同波段下测量光谱与模拟光谱的相对误差

Table1. Relative error between measured and calculated optical density spectra in different bands

表 2250~750 nm波段范围内大肠杆菌透射光谱模型解析参数

Table2. Calculated parameters from the transmittance model in 250-750 nm band for Escherichia coli

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水体细菌微生物多波长透射光谱解析模型下载： 856次