为克服十二烷基苯磺酸钠与甜菜碱之间的胶束化作用及相互协同作用对二者定量分析产生的干扰, 按十二烷基苯磺酸钠的摩尔计量比加入1∶1的羟丙基-β-环糊精, 分别采用同步荧光光谱法及“化学-光谱”分析法检测复配体系中二者含量.结果表明:十二烷基苯磺酸钠的临界胶束浓度值随甜菜碱浓度的增加而降低, 当甜菜碱的浓度增加到1.0、2.0及3.0 mmol/L时, 十二烷基苯磺酸钠的临界胶束浓度由1.256 mmol/L分别降低至1.121、0.989及0.684 mmol/L; 甜菜碱还能对低浓度十二烷基苯磺酸钠溶液(浓度低于临界胶束浓度)的同步荧光强度产生明显影响.另一方面, 十二烷基苯磺酸钠对“化学-光谱”分析法检测甜菜碱也能产生明显干扰.加入羟丙基-β-环糊精后, 能显著降低十二烷基苯磺酸钠和甜菜碱之间的相互干扰, 复配体系中十二烷基苯磺酸钠和甜菜碱的回收率分别由79.7%~107.5%及119.7%~159.6%改变至101.5%~102.5%和101.5%~103.0%.

In order to decrease the influence caused by the micellization and the interaction between Sodium Dodecyl Benzene Sulfonate (SDBS) and betaine on determination of SDBS or betaine in aqueous solution. Synchronous fluorescence spectra and spectrochemical method were utilized to analyze the concentrations of SDBS and betaine by adding 1∶1 (molar ratio) hydroxypropyl-β-cyclodextrin (HP-β-CD) according to the amount of SDBS in their complex aqueous solution.The results indicate that the Critical Micelle Concentration (CMC) values of SDBS could be greatly increased with increasing betaine concentration in SDBS and betaine complex aqueous solution. The CMC values of SDBS reduced from 1.256 mmol/L to 1.121, 0.989 and 0.684 mmol/L when the concentrations of betaine respectively increased from 0 to 1.0, 2.0 and 3.0 mmol/L in their complex aqueous solution. The synchronous fluorescence intensities of SDBS could also be easily affected by betaine even in low concentration SDBS solution (the concentration is lower than CMC). Meanwhile, effect of the interferences on the quantitative determination of betaine by spectrochemical method could be obviously increased in the presence of SDBS. Both the interferences of betaine on synchronous fluorescence spectrum of SDBS and the interferences of SDBS on the spectrochemical determination of betaine, could be greatly reduced by adding 1:1 HP-β-CD. The recovery rates of SDBS and betaine respectively changed from 104.2%~109.2% and 119.7%~159.6% to 101.5%~102.5% and 101.5%~103.0% in their binary complex aqueous solution.