高速扫描激光共聚焦显微内窥镜图像校正

徐宝腾; 杨西斌; 刘家林; 周伟; 田浩然; 熊大曦

doi:doi:10.3788/ope.20202801.0060

光学精密工程, 2020, 28 (1): 60, 网络出版: 2020-03-25

高速扫描激光共聚焦显微内窥镜图像校正

Image correction for high speed scanning confocal laser endomicroscopy

论文大纲

徐宝腾 ^*杨西斌刘家林周伟田浩然熊大曦

作者单位

中国科学院苏州生物医学工程技术研究所光与健康研究中心, 江苏苏州 215163

激光共聚焦显微内窥镜畸变校正错位校正互相关法遗传算法 confocal laser endoscope distortion correction dislocation correction cross-correlation genetic algorithm

摘要

使用往复式逐行扫描的方式可以提高激光共聚焦显微内窥镜的成像速度和数据利用率, 但这种扫描方式也会带来图像畸变和错位问题, 从而影响系统成像质量。受扫描畸变影响, 图像错位程度不一致, 后期处理难以得到理想的校正效果。本文基于共振振镜的运动规律, 推导了均匀空间采样过程中的采样时间函数, 通过非等时采样方法校正了振镜速度变化带来的横向畸变。利用互相关法评价图像错位程度, 采用遗传算法获得最优的采样开始时刻, 实现了图像错位的校正。最终通过调整采样开始时刻和时间间隔在数据采集环节校正了图像畸变和错位。为了验证图像畸变校正和错位校正效果, 本文搭建了基于往复式逐行扫描方式的激光共聚焦显微内窥成像系统。实验结果表明, 该方法能够有效地校正图像畸变和错位, 图像的横向分辨率。与现有方法相比, 本文方法将图像的局部分辨率由10 pixel提高到6 pixel。

Abstract

Reciprocating progressive scanning can improve the imaging speed and data utilization of confocal laser endomicroscopy.However, this scanning method can also create image distortion and dislocation, which affects the imaging quality of the system. In this study, the sampling time function during the anisochronous sampling process is deduced based on the movement rule of the galvanometric resonance scanner, and the horizontal distortion caused by speed changes in the galvanometric scanner is corrected. Moreover, the cross-correlation method is used to assess the degree of image dislocation. The genetic algorithm is used to obtain the optimal starting time of the sampling, which results in the correction of the image dislocation. Finally, the image distortion and dislocation are corrected by adjusting the sampling start time and time interval of the data acquisition.The confocal endomicroscopic imaging system based on reciprocating progressive scanning is established to verify the effects of correction of image distortion and dislocation. Experimental results show that this method can effectively correct image distortion and dislocation, and further improve the lateral resolution of images.Compared with existing methods, the local resolution of the image corrected by the method in this paper is improved from 10 pixel to 6 pixel.

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徐宝腾, 杨西斌, 刘家林, 周伟, 田浩然, 熊大曦. 高速扫描激光共聚焦显微内窥镜图像校正[J]. 光学精密工程, 2020, 28(1): 60. XU Bao-teng, YANG Xi-bin, LIU Jia-lin, ZHOU Wei, TIAN Hao-ran, XIONG Da-xi. Image correction for high speed scanning confocal laser endomicroscopy[J]. Optics and Precision Engineering, 2020, 28(1): 60.

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