光学学报, 2009, 29 (7): 1921, 网络出版: 2009-07-20   

噻酮提高在体大鼠皮肤光透明效果的实验研究

Experimental Study on in vivo Rat Skin Optical Clearing at the Action of Thiazone
作者单位
华中科技大学武汉光电国家实验室(筹)Britton Chance生物医学光子学研究中心, 湖北 武汉 430074
摘要
为探讨在体皮肤实验中最为简便有效的给药方式, 将化学渗透促进剂噻酮与聚乙二醇400(PEG400)的混合溶液直接作用于去除部分角质和含角质的在体大鼠皮肤, 利用CCD拍照进行直观观察, 并通过反射光谱的变化来反映皮肤光透明效果, 同时与单纯PEG400作用进行了对比。结果表明:混合溶液可以使去除部分角质皮肤在15 min内反射光谱明显下降, 并产生显著的光透明效果, 30 min后皮肤更加透明; 而单纯PEG400以及未去除角质层的完整皮肤均无光透明效果。因此, 结合物理方法去除部分角质层、并添加促渗剂噻酮, 能以非侵入方式, 快速、有效提高PEG400对在体皮肤的光透明效果。
Abstract
In quest of the most simple and effective treatment for in vivo skin experiments, thiazone was used as an enhancer in our study. To compare with the pure polyethyleneglycol 400 (PEG400) solution, the mixed solution (thiazone in combination with) was applied on the stratum corneum partly removed skin and intact skin respectively. For visual observation, photographs of the local skins were taken by CCD; and then the light reflectance spectra of skin were monitored. The results showed that a mixed solution could make the skin without stratum corneum more transparent in 15 min, with the reflectance spectrum decreased significantly; while there’s no observable change in the intact skins. However, the pure PEG400 did not have optical clearing effects on any skins. In conclusion, the non-invasive drug delivery method, which is combined with physically abrading part of the stratum corneum and adding chemical enhancer-thiazone, can improve the effect of PEG400 in skin optical clearing quickly and effectively.
参考文献

[1] . V. Tuchin. Optical immersion as a new tool for controlling the optical properties of tissues and blood[J]. Laser Physics, 2005, 8(15): 1109-1136.

[2] . Vargas, K. F. Chan, S. L. Thomsen et al.. Use of osmotically active agents to alter optical properties of tissue: effects on the detected fluorescence signal measured through skin[J]. Lasers Surg. Med., 2001, 29(3): 213-220.

[3] . Mao, D. Zhu, Y. Hu et al.. Influence of alcohols on the optical clearing effect of skin in vitro[J]. J. Biomed. Opt., 2008, 13(2): 021104.

[4] . Zhu, H. Cui, J. Zhang et al.. Transient response and hysteresis of blood vessel in chick chorioallantoic membrane by hyperosmotic agents[J]. J. Biomed. Opt., 2008, 13(2): 021106.

[5] . H. Khan, B. Choi, S. Chess et al.. Optical clearing of in vivo human skin: implications for light-based diagnostic imaging and therapeutics[J]. Lasers Surg. Med., 2004, 34(2): 83-85.

[6] . V. Tuchin. Optical clearing of tissues and blood using the immersion method[J]. J. Phys. D: Appl. Phys., 2005, 38(7): 2497-2518.

[7] 岑建, 朱丹, 骆清铭 等. 渗透剂对小鼠皮肤光学特性影响的实时动态监测[J]. 光学学报, 2004, 24(7):873~876

    Chen Jian, Zhu Dan, Luo Qingming et al.. Monitoring osmotical agents induced the change in optical properties of mouse skin [J]. Acta Optica Sinica, 2004, 24(7):873~876

[8] . Y. Jiang, R. K. Wang. Comparing the synergistic effects of oleic acid and dimethyl sulfoxide as vehicles for optical clearing of skin tissue in vitro[J]. Phys. Med. Biol., 2004, 49(23): 5283-5294.

[9] . Choi, L. Tsu, E. Chen et al.. Determination of chemical agent optical clearing potential using in vitro human skin[J]. Lasers Surg. Med., 2005, 36(2): 72-75.

[10] . H. Khan, S. Chess, B. Choi et al.. Can topically applied optical clearing agents increase the epidermal damage threshold and enhance therapeutic efficacy[J]. Lasers Surg. Med., 2004, 35(2): 93-95.

[11] . I. Galanzha, V.V.Tuchin, A.V. Solovieva et al.. Skin back reflectance and microvascular system functioning at the action of osmotic agents[J]. J. Phys. D: Appl. Phys., 2003, 36(14): 1739-1746.

[12] . Vargas, E. K. Chan, J. K. Barton et al.. Use of an agent to reduce scattering in skin[J]. Lasers Surg. Med., 1999, 24(2): 133-141.

[13] . F. Stumpp, B. Chen, A. J. Welch. Using sandpaper for noninvasive transepidermal optical skin clearing agent delivery[J]. J. Biomed. Opt., 2006, 11(4): 041118.

[14] . V. Tuchin, G. B. Altshuler, A. A. Gavrilova et al.. Optical clearing of skin using flashlamp- induced enhancement of epidermal permeability[J]. Lasers Surg. Med., 2006, 38(9): 824-836.

[15] . 新型促渗剂噻酮[J]. 精细与专用化学品, 2004, 12(21): 9-11.

    . A new skin penetration promoter: thiazone[J]. Fine and Specialty Chemicals, 2004, 12(21): 9-11.

[16] 毛宗珍, 胡亚婷, 郑英 等. 噻酮对离体乳猪皮肤光透明效果影响的实验研究[J]. 光学学报, 2007, 27(3):526~530

    Mao Zongzhen, Hu Yating, Zheng Ying et al.. Experimental study of influence of thiazone on optical clearing of piglet skin in vitro[J]. Acta Optica Sinica, 2007, 27(3):526~530

[17] 郑俊民. 经皮给药新剂型[M]. 北京:人民卫生出版社, 1997, 36~37

    Zheng Junmin. New Transdermal Deliver Preparations [M]. Beijing: People’s Medical Publishing House, 1997, 36~37

[18] 朱盛山. 药物新剂型[M]. 北京:化学工业出版社, 2003, 306~307

    Zhu Shengshan. New Form of Prepared Drugs [M]. Beijing: Chemical Industry Press, 2003, 306~307

[19] . Scheindlin. Transdermal drug delivery: past, present, future[J]. Mol. Interv., 2004, 4(6): 308-312.

毛宗珍, 韩珍珍, 朱丹. 噻酮提高在体大鼠皮肤光透明效果的实验研究[J]. 光学学报, 2009, 29(7): 1921. Mao Zongzhen, Han Zhenzhen, Zhu Dan. Experimental Study on in vivo Rat Skin Optical Clearing at the Action of Thiazone[J]. Acta Optica Sinica, 2009, 29(7): 1921.

本文已被 1 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!