光子学报, 2018, 47 (4): 0422003, 网络出版: 2018-03-15  

利用MAP评估提高表面等离子体结构光照明技术成像质量

Improving the Imaging Performance of Plasmonic Structured Illumination Microscopy Using MAP Estimation Method
作者单位
中国科学院长春光学精密机械与物理研究所 应用光学国家重点实验室,长春 130033
摘要
经典的恢复算法不能有效地恢复被观测物的全频域信息, 空间频率的缺失导致超分辨图像伴有较为严重的旁瓣.本文提出利用最大后验概率(MAP)评估解决表面等离子体结构光照明技术中的光学旁瓣问题.结果表明MAP评估恢复算法可以有效恢复物质的高空间频率信息, 并且通过合理选择优化参数达到抑制光学旁瓣的目的.在波长520 nm, 数值孔径1.3下, 可获得半高全宽65 nm的横向分辨力, 约为传统荧光显微镜的3.6倍.该技术在生命科学观测中具有潜在应用价值.
Abstract
The classical restoration algorithm can not effectively recover the full frequency domain information of the object, which leads to the serious optical sidelobes. In this paper, the application of metal nano arrays in structured light illumination is studied, and the problem of optical sidelobes in plasmonic structured illumination microscopy is solved using Maximum A Posteriori (MAP) estimation. The research shows that the MAP estimation method can effectively restore the high spatial frequency information, and through the reasonable selection of optimization parameters to achieve the purpose of suppressing optical sidelobes. At the wavelength of 520 nm, 1.3 numerical aperture, the lateral resolution can be obtained at FWHM of 65 nm, which is about 3.6 times of the traditional fluorescence microscope. This technology has potential application application in the field of life science.
参考文献

[1] ABBE E. Beitrge zur theorie des mikroskops und der mikroskopischen wahrnehmung[J]. Archiv FR Mikroskopische Anatomie, 1873, 9(1): 413-418.

[2] GUSTAFSSON M G L. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy[J]. Journal of Microscopy, 2000, 198(2): 82-87.

[3] HEINTZMANN R, CREMER C. Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating\[C\]. SPIE. 1999, 3568(185): 15.

[4] FROHN J T, KNAPP H F, STEMMER A. True optical resolution beyond the Rayleigh limit achieved by standing wave illumination[J].Proceedings of the National Academy of Sciences, 2000, 97(13): 7232-7236.

[5] HEINTZMANN R, JOVIN T M, CREMER C. Saturated patterned excitation microscopy—a concept for optical resolutionimprovement[J]. Journal of the Optical Society of America A, 2002, 19(8): 1599-1609.

[6] SCHERMELLEH L, CARLTON P M, HAASE S, et al. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy[J]. Science, 2008, 320(5881): 1332-1336.

[7] KNER P, CHHUN B B, GRIFFIS E R, et al. Super-resolution video microscopy of live cells by structured illumination[J]. Nature Methods, 2009, 6(5): 339-342.

[8] WEI F, LIU Z. Plasmonic structured illumination microscopy[J]. Nano Letters, 2010, 10(7): 2531-2536.

[9] CAO S, WANG T, XU W,et al. Gradient permittivity meta-structure model for wide-field super-resolution imaging with a sub-45 nm resolution[J]. Scientific Reports, 2016, 6.

[10] PONSETTO J L, WEI F, LIU Z. Localized plasmon assisted structured illumination microscopy for wide-field high-speed dispersion-independent super resolution imaging[J]. Nanoscale, 2014, 6(11): 5807-5812.

[11] CAO S, Wang T, SUN Q, et al. Meta-nanocavity model for dynamic super-resolution fluorescent imaging based on the plasmonic structure illumination microscopy method[J]. Optics Express, 2017, 25(4): 3863-3874.

[12] LAl A, SHAN C, XI P. Structured illumination microscopy image reconstructionalgorithm[J]. IEEE Journal of Selected Topics in Quantum Electronics, 2016, 22(4): 50-63.

[13] LI D, SHAO L, CHEN B C, et al. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics[J]. Science, 2015, 349(6251): aab3500.

[14] 文刚,李思黾,杨西斌,等. 基于激光干涉的结构光照明超分辨荧光显微镜系统[J]. 光学学报,2017,37(3): 25-35.

    WEN G, LI S, YANG X, et al. Super-resolution fluorescence microscopy system by structured light illumination based on laser interference[J]. Acta Optica Sinica, 2017, 37(3): 25-35.

[15] CHANG B J, CHOU L J, CHANG Y C, et al. Isotropic image in structured illumination microscopy patterned with a spatial light modulator[J]. Optics Express, 2009, 17(17): 14710-14721.

[16] BRONGERSMA M L, KIK P G. Surface plasmon nanophotonics[M]. Springer, 2007.

[17] 宋超,郝鹏,余幕欣,等. 金纳米线与亚波长狭缝结合实现局域场增强研究[J]. 光子学报,2014,43(1): 0116001.

    SONG C, HAO P, YU M, et al. Enhancing electric field with gold subwavelength slits and nanowires[J]. Acta Photonica Sinica, 2014, 43(1): 0116001.

[18] FANG N, LEE H, SUN C, et al. Sub-diffraction-limited optical imaging with a silver superlens[J]. Science, 2005, 308(5721): 534-537.

[19] MILANFAR P, Super-resolution imaging[M]. CRC press, 2010.

[20] FRANOIS O, EDUARDO S, VINCENT L,et al. Bayesian estimation for optimized structured illumination microscopy[J]. IEEE Transactions on Image Processing, 2012, 21(2): 601-614.

[21] TOMAS L, PAVELKR Z, ZDENEK S, et al. Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation[J]. Optics Express, 2014, 22(24): 29805-29817.

[22] YOSHIKAWA H, ADACHI S. Optical constants of ZnO[J]. Japanese Journal of Applied Physics, 1997, 36(10R): 6237.

[23] JOHNSON P B, CHRISTY R W. Optical constants of the noble metals[J]. Physical Review B, 1972, 6(12): 4370.

[24] LI L. New formulation of the Fourier modal method for crossed surface-relief gratings[J]. Journal of the Optical Society of America A, 1997, 14(10): 2758-2767.

[25] WHITE J S, VERONIS G, YU Z, et al. Extraordinary optical absorption through subwavelength slits[J]. Optics Letters, 2009, 34(5): 686-688.

[26] WRIGHT S J, NOCEDAL J. Numerical optimization[M]. Springer Science, 1999, 35(67-68): 7.

[27] RAINER H, PIER A. B. High-resolution reconstruction in fluorescence microscopy with patterned excitation[J]. Applied Optics, 2006, 45(20): 5037-5045.

余慕欣, 周文超, 吴一辉. 利用MAP评估提高表面等离子体结构光照明技术成像质量[J]. 光子学报, 2018, 47(4): 0422003. YU Mu-xin, ZHOU Wen-chao, WU Yi-hui. Improving the Imaging Performance of Plasmonic Structured Illumination Microscopy Using MAP Estimation Method[J]. ACTA PHOTONICA SINICA, 2018, 47(4): 0422003.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!