氯胺酮对He-Ne激光照射促人正常皮肤成纤维细胞增殖作用的影响

针对动物模型伤口的光生物调节作用,用He-Ne激光对人正常皮肤成纤维细胞增殖的促进作用作为细胞模型,研究了麻醉药氯胺酮的影响。用MTT法测定细胞增殖作用,分别研究了1.56 mW/cm2 He-Ne激光(0,10,30,100和300 s照射)对人正常皮肤成纤维细胞的增殖作用和氯胺酮(0,0.6,1.2和2.0 μg/mL)预处理对He-Ne激光(300 s照射)细胞增殖作用的影响。氯胺酮(0.6,1.2,2.0 μg/mL)预处理能抑制468.7 mJ/cm2 He-Ne激光促人正常皮肤成纤维细胞增殖的作用。这说明动物实验中用于麻醉的药物可能对激光促进伤口愈合的实验结果有影响。

Abstract

Based on the photobiomodulation on wound healing of animal models, the stimulative effects of He-Ne laser irradiation on proliferation of human normal skin fibroblasts were used as the cell model to investigate the role of ketamine as an anesthetics in animal model experiments in this paper. The cell proliferation was measured using MTT-based colorimetric assay. The effects of 1.56 mW/cm2 He-Ne laser irradiation on human normal skin fibroblasts for 0, 10, 30, 100, 300 s, and on the cells preincubated with different concentrations of ketamine (0, 0.6, 1.2, 2.0 μg/mL) for 300 s were investigated, respectively. The results show that human normal skin fibroblast proliferation induced by He-Ne laser irradiation (468.7 mJ/cm2) was inhibited when the cells were preincubated with different concentrations of ketamine (0.6, 1.2, 2.0 μg/mL). These results suggest that anesthetics may affect photobiomodulation on wound healing.

参考文献

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李燕, 角建瓴, 刘承宜, 洪岸, 刘颂豪. 氯胺酮对He-Ne激光照射促人正常皮肤成纤维细胞增殖作用的影响[J]. 中国激光, 2004, 31(4): 509. 李燕, 角建瓴, 刘承宜, 洪岸, 刘颂豪. Effects of Ketamine in Combination with 468.7 mJ/cm2 He-Ne Laser Irradiation on Proliferation in Human Skin Fibroblasts[J]. Chinese Journal of Lasers, 2004, 31(4): 509.

氯胺酮对He-Ne激光照射促人正常皮肤成纤维细胞增殖作用的影响

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