黄牛(Bos taurus)基因组P450基因家族的分析

利用黄牛基因组cDNA和氨基酸数据库对P450基因进行搜索和分析, 结果显示: 在黄牛基因组中发现64个P450基因, 分别属于18个P450家族和38个亚家族。以氨基酸序列相似度大于60 %为标准对黄牛P450基因进行分组, 32个为孤儿基因, 其余32个可归入9个组, 其中7个组适合于正选择和基因转换分析。结果表明: 有3个组显著受到正选择压力作用, 其中的2个组有正选择概率大于95 %的氨基酸位点, 分别位于底物识别位点SRS1和SRS2; 有5个组显示显著的基因转换事件; 既显著受到正选择压力作用, 又参与基因转换的基因共4个。可见, 发生基因转换的基因与受到显著正选择的基因有一定的相关性。此外, 鉴定出20个不同的基序, 其中有6条基序在90 ％以上的基因中出现。

Abstract

A genome-wide analysis of P450 genes were performed in cow genome based on their cDNA and amino acid data. Sixty-four P450 genes, assigned into 18 gene families and 38 subfamilies, were identified. All P450 genes were partitioned into 9 groups and 32 orphan genes by a criterion that the genes belong to a group when their amino acid identity were more than 60 %. Positive selection and gene conversion were analyzed in other 7 groups. The results showed that positive selections were detected in 3 groups. The positively selected sites, locating in SRS (substrate recognition sites) 1 and SRS 2, were detected in 2 groups with a more than 95 % probability. And gene conversions were detected in 5 groups. Interestingly, both positive selection and gene conversion events were identified obviously in 4 genes. The certain correlation was revealed between positive selection and gene conversion. Moreover, twenty motifs were identified, thereinto, 6 motifs existed in over 90 % of P450 genes.

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陈丽娟, 程茂基, 张云华. 黄牛(Bos taurus)基因组P450基因家族的分析[J]. 激光生物学报, 2010, 19(1): 61. CHEN Li-juan, CHENG Mao-ji, ZHANG Yun-hua. A Genome-wide Analysis of P450 Gene Family in Cow (Bos taurus)[J]. Acta Laser Biology Sinica, 2010, 19(1): 61.

黄牛(Bos taurus)基因组P450基因家族的分析

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