He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复

姜晓燕; 高丽美; 李永峰; 韩榕

doi:doi:10.3788/gzxb20114005.0712

光子学报, 2011, 40 (5): 712, 网络出版: 2011-06-14

He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复

Ramage Repair for Enhanced Ultraviolet-B Radiation on Antioxidant System of Arabiodopsis by He-Ne Laser

论文大纲

姜晓燕 ^*高丽美李永峰韩榕

作者单位

山西师范大学生命科学学院，山西临汾 041004

He-Ne激光 UV-B辐射拟南芥抗氧化系统 He-Ne laser UV-B radiation Arabidopsis Antioxidant systems

摘要

以培养6周左右的拟南芥为材料，采用UV-B辐射（剂量1 KJ/m2/d）和He-Ne激光器（波长632.8 nm,输出功率5 mW·mm2,辐照时间60 s）对材料进行处理，分成CK（没有经过UV-B或激光辐照）组、B(UV-B辐射)组、BL（UV-B和激光复合处理）组和L（激光辐照）组4个不同处理组.结果表明：增强的UV-B辐射拟南芥幼苗导致MDA(Malondialdehyde)、超氧阴离子含量升高，GSH(Glutathione)含量降低，PAL(phenylalanine ammomia-lyase)、CAT(catalase)和APX(ascorbate peroxidase)活性升高，SOD(supemxide dismutas)活性降低. 单独He-Ne激光处理使MDA、超氧阴离子含量降低，GSH的含量升高，SOD、APX、CAT的活性升高，PAL的活性降低.UV-B辐射后再用He-Ne激光进行后处理，发现与单独UV-B辐照处理相比，MDA、超氧阴离子含量降低，GSH含量升高，SOD、APX、CAT的活性升高了，PAL的活性降低了.因此激光在一定程度上提高了拟南芥叶片抗氧化能力，在此基础上讨论了其可能的形成机理.

Abstract

Taking Arabidopsis trained about six weeks as the material,UV-B radiation(1 kJ/m2/d) and He-Ne laser irradiation(wavelength 632.8 nm output power 5 mW·mm2, irradiation time 60 s) were used to process the material.The Arabidopsis were divided into four treatment groups including CK(not treated ),L(He-Ne laser) ,B(UV-B), and BL(UV-B +He-Ne laser).The experimental results show that enhanced UV-B increases the contents of MDA,O.－2and the activities of PAL, CAT,APX ,while SOD activity and the content of GSH decrease. But He-Ne laser irradiation reduces the contents of MDA and O.－2,the PAL activity, and increases SOD activity, APX activity, CAT activity, the content of GSH .In contrast to B group, both enhanced UV-B and He-Ne laser have similar results which indicated by He-Ne laser irradiation. The results prove that laser can improve the ability of antioxidant system in Arabidopsis.

参考文献

[1] 陈浩.UV-B胁迫对烟草生长发育及生理生化特性影响的研究［D］.合肥：安徽农业大学,2009:1-42.

CHEN Hao. UV-B radiation on the impact of growth and physiological and biochemical characteristics［D］. Hefei: Anhui Agricultural University, 2009: 1-42.

[2] 龙云,刘芸,钟章成,等.酸雨和UV-B对玉米幼苗光合速率和抗氧化酶活性的影响［J］.生态学报,2009,29（9）:4957-4966.

LONG Yun, LIN Yun, ZHONG Zhang-cheng, et al. Effect of UV-B irradiation and acid rain on photosynthetic rate and antioxidant enzyme activities of maize seedings［J］. Acta Ecologica Sinica, 2009, 29(9): 4957-4966.

[3] 张新永,郭华春,戴华峰.增强UV-B辐射对彩色马铃薯叶片中相关保护酶活性的影响［J］.西北植物学报,2009,29（5）:0968-0974.

ZHANG Xin-yong, GUO Hua-chun, DAI Hua-feng. Effect of supplement ultraviolat-b on the related protective enzymes in the leaves of chromatic potato［J］. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(5): 0968-0974.

[4] 刘清华,钟章成.紫外线-B辐射对银杏活性氧代谢及膜系统的影响［J］.西北师范大学学报,2007,28(2):149-153.

LIU Qing-hua, ZHONG Zhang-cheng. Effect of UV-B on ROS metabolism and membrane system［J］. Journal of China West Normal University, 2007, 28(2): 149-153.

[5] 赵广琦.增强UV-B辐射和CO2倍增的复合作用对蚕豆、小麦生长和生理生态的影响［D］.西安:西北大学,2003:1-82.

ZHAO Guang-qi. The combined effects of enhanced UV-B irradiation and doubled CO2 on the growth and physioecology of broa bean and wheat［D］. Xi′an: Northwestern University, 2003: 1-82.

[6] 韩榕.He-Ne激光对小麦增强UV-B辐射损伤的修复效应及机理［D］.西安:西北大学,2002:1-81.

HAN Rong. Mechanism and repair effect of Wheat seedlings exposed to enhanced ultraviolet-B radiation by He-Ne laser［D］. Xi′an: Northwestern University, 2002: 1-81.

[7] 张娟,韩榕. He-Ne激光对增强UV-B辐射小麦细胞染色体损伤的影响［J］.山西师范大学学报,2008,22(2):67-70.

ZHANG Juan, HAN Rong. The influence of he-ne laser irradiation on the damage of cell chromosome in wheat by enhanced UV-B radiation［J］. Journal of ShanXi Teachers University, 2008, 22(2): 67-70.

[8] 郭君瑜,张琴,韩榕.He-Ne激光对增强UV-B辐射小麦幼苗叶绿体的影响［J］.植物研究,2009,29（2）:204-208.

GUO Jun-yu, ZHANG Qin, HAN Rong. Effects of He-Ne laser on chloroplast of wheat seedling exposed to enhanced UV-B radiation［J］. Bulletin of Botanical Research, 2009, 29(2): 204-208.

[9] 孔祥生,易现峰.植物生理学实验技术［M］.北京:中国农业出版社,2008:257-259.

[10] 孙群,胡景江.植物生理学研究技术［M］.杨凌:西北农林科技大学出版社,2006.

[11] 李玲等.植物生理学模块实验指导［M］.北京:科学出版社,2009:100-102.

[12] 郝金花,马晓丽,韩榕等.He-Ne激光对UV-B辐射小麦幼苗抗氧化系统的影响［J］.武汉植物学研究,2007,25（4）: 350-355.

HAO Jin-hua, MA Xiao-li, HAN Rong, et al. Effects of He-Ne laser on antioxidant systems of wheat seedlings exposed to ultraviolet-b radiation［J］. Journal of Wuhan Botanical Rasearch, 2007, 25(4): 350-355.

[13] 郭炜.紫外辐照导致植物细胞DNA损伤的彗星电泳检测及生理指标的测定［D］.济南:山东大学,2008:1-93.

GUO Wei. Evaluation of UV damage at DNA level in several plant protoplasts using SCGE and UV resistant physiological indicators detecting ［D］ . Jinan: Shandong University, 2008: 1-93.

[14] 魏金凤,郭敬功,张清德等.UV辐射对拟南芥叶片抗氧化酶系统和膜系统的影响［J］ .河南大学学报,2009,39（5）: 501-504.

WEI Jin-feng, GHO Jing-gong, ZHANG Qing-de, et al. Effect of ultraviolet radiation on the antioxidant enzymes and the membrane systems of arabidopsis thaliana leaves［J］. Journal of Henan University, 2009, 39(5): 501-504.

[15] 李冉,杨薇,王玉洁.崧蓝幼苗对激光和增强UV-B辐射的生理响应研究［J］.应用激光,2009,29（2）:158-160.

LI Ran, YANG Wei, WANG Yu-jie. Physiological response of lsatis indigotica seedlings subjected to elevated UV-B to laser radiation［J］. Applied Laser, 2009, 29(2): 158-160.

[16] 齐智.激光对增强的UV-B辐射蚕豆幼苗损伤的防护及修复作用［D］.西安:西北大学,2001:1-63.

QI Zhi. Repair and protection on enhanced UV-B on broad bean seedlings by He-Ne laser［D］. Xi′an: Northwestern University, 2001: 1-63.

[17] 向洋,丁志宝.激光生物学作用机理探讨［J］.光电子·激光,1997,8（6）:476-478.

XIANG Yang, DING Zhi-bao. The inquiry in effect mechanism of laser biology［J］. Optoelectronics·Laser, 1997, 8(6): 476-478.

姜晓燕, 高丽美, 李永峰, 韩榕. He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复[J]. 光子学报, 2011, 40(5): 712. JIANG Xiao-yan, GAO Li-mei, LI Yong-feng, HAN Rong. Ramage Repair for Enhanced Ultraviolet-B Radiation on Antioxidant System of Arabiodopsis by He-Ne Laser[J]. ACTA PHOTONICA SINICA, 2011, 40(5): 712.

He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复

关于本站 Cookie 的使用提示

全站搜索

He-Ne激光对增强UV-B辐射拟南芥抗氧化系统的损伤修复

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索