油菜种子的油脂和蛋白积累及相关基因表达的研究

检测了油菜籽成熟过程的总蛋白和粗脂肪含量变化, 以及与之相关23个基因在各器官及果荚各发育时期的表达情况。结果显示: 蛋白在开花后25天(25 DAF)后大量累积, 而油脂则在25 DAF后增速放缓, 30 DAF后才急剧上升。多数油脂合成相关酶基因在果荚中表达最高, 叶片中次之, 花蕾和花朵中有少量表达, 茎中的表达很低, 少数在根中表达, 部分基因的表达随油菜果荚发育而持续增加, 另一部分则呈先升高, 再下降至25 DAF低点, 再回升的趋势; 转录因子LEC1、LEC2及WRI与贮藏蛋白基因Napin、Cruciferin主要在果荚中表达, 前者的表达主要在果荚发育前期大量, 其中WRI的出现起伏波动, 后者主要在25、30 DAF大量表达。这表明种子成熟时总蛋白和粗脂肪含量变化可能与相关基因表达变化相关。

Abstract

The content of total protein and crude fat in seed was detected during different stages of seed filling in oilrape, and the expression profiles of 23 related genes were determined in different organs and different development stages siliques. The result shows that protein content increased sharply after 25 days after flowering (DAF), while the increase of oil content slowed down at 25 DAF but accelerated greatly after 30 DAF. The transcript level of most oil-synthesis enzyme genes were expressed highest in siliques, then lower in leaves, and less in buds and flowers, and lowest in stems, few of these genes were expressed in roots. The transcript profiles of these genes would be divided into two patterns during seed filling: one increased standing; the other one increased at the beginning, then slowed down until 25 DAF, and then increased again until the end. Transcript factors (LEC1, LEC2 and WRI) and storage protein genes (Napin, Cruciferin) were mainly expressed in siliques, and the former were mainly expressed during earlier stage of siliques maturing and WRI expressed in waved curve over the time, the latter were strongly expressed at 25, 30 DAF. This suggests that the changes of seed total protein and crude fat content during seed filling would correlate with the expression changes of involved genes.

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