中国激光, 1991, 18 (4): 313, 网络出版: 2007-11-12
激光微束显微切割植物染色体的研究
Study on laser micro-dissection of chromosome in plants
摘要
Abstract
Chromosome samples were prepared from dividing cells of root topa of broad bean, maize, wheat, and barley, etc. by means of aquash method. Miero-disseetion of chromosome was performed on the microscopic stage using argon laser microirradiation (514.5 nm). The chromosomes could be dissected into 12,6-9,6 and 4-6 chromosomal fractions respectively while the suitable laser power densities were applied. The establishment of laser micro-dissection of chromosome in plants provides the possibillity of the application of laser microbeam irradiation in chromosome engineering, assignment of genes, and DNA micro-cloning from chromosomal fragment in crons.
参考文献
王兰岚, 陈仲康, 黄力全, 宋桂英, 徐正平, 梁宏. 激光微束显微切割植物染色体的研究[J]. 中国激光, 1991, 18(4): 313. 王兰岚, 陈仲康, 黄力全, 宋桂英, 徐正平, 梁宏. Study on laser micro-dissection of chromosome in plants[J]. Chinese Journal of Lasers, 1991, 18(4): 313.