复合纳米颗粒中白藜芦醇-大麦醇溶蛋白相互作用研究

为阐明大麦醇溶蛋白包覆白藜芦醇形成纳米颗粒的分子机制, 采用荧光光谱、紫外吸收光谱以及傅里叶红外光谱和差示扫描量热技术等研究了白藜芦醇与大麦醇溶蛋白分子间的相互作用。结果表明: 白藜芦醇与大麦醇溶蛋白分子的相互作用, 对其内源性荧光具有猝灭作用, 结合差示扫描量热的结果, 推测二者通过静态猝灭过程形成了新的复合物; 计算出两者之间的结合常数与结合位点数分别为KA(298 K)=2.21×105 L·mol-1, KA(310 K)=1.53×104 L·mol-1和n298 K=1.23, n310 K=0.94; 依据热力学参数和傅里叶红外光谱判断白藜芦醇与大麦醇溶蛋白之间的作用力为范德华力和氢键相互作用; 根据Frster非辐射能量转移原理, 计算出白藜芦醇与大麦醇溶蛋白的结合距离为3.25 nm, 能量转移效率为0.227; 同步荧光检测结果表明白藜芦醇结合大麦醇溶蛋白分子后对其构象产生了明显影响, 且该影响是由色氨酸残基所处环境的疏水性变化所致。

Abstract

In order to clarify the interaction mechanism of the formation of the resveratrol-hordein nanoparticle, the fluorescence, UV-Vis spectroscopic, FTIR and DSCwere used to study the binding reaction between resveratrol and hordein. The fluorescent emission of hordein was inhibited by resveratrol in a dose dependent manner. Fluorescence spectroscopy and DSC indicated that resveratrol interacted with hordein and formed a new complex by a static process. The binding constant(KA(298 K)=2.21×105 L·mol-1, KA(310 K)=1.53×104 L·mol-1) and the number of binding sites(n298 K=1.23, n310 K=0.94)were calculated based on the quenching effect of resveratrol on hordein. Thermodynamic parameter and FTIR indicated that the interaction force between resveratrol and hordein was mainly hydrogen binding and van der Waals force. The binding distance(r0=3.25 nm)between resveratrol and hordein and the energy transfer efficiency (E=0.227) were obtained according to non-radiative energy transfer theory. The effect of resveratrol on the conformation of hordein was further analyzed by using synchronous fluorescence spectrometry. The results indicated that resveratrol changed the hydrophobicity of tryptophan residue, which caused an obvious influence on the conformation of hordein.

参考文献

[1] HAN Jing-jing, LIU Wei, BI Yu-ping(韩晶晶, 刘炜, 毕玉平). Chinese Journal of Biotechnology (生物工程学报), 2008, 24(11): 1851.

[2] YIN Cheng-xia(印成霞). Guide of China Medicine(中国医药指南), 2013, (23): 362.

[3] GAO Jie, LIAN Xiao-yan, WEI Zhen-ping, et al(高洁, 连潇嫣, 魏振平, 等). Chemical Industry and Engineering(化学工业与工程), 2012, 29(5): 64.

[4] Dube A, Nicolazzo J A, Larson I. European Journal of Pharmaceutical Sciences, 2010, 41(2): 219.

[5] Patel A R, Heussen P C M, Hazekamp J, et al. Food Chemistry, 2012, 133(2): 423.

[6] LIU Gui-jin, WANG Hong-di, JIANG Yan-bin(刘贵金, 汪红娣, 江燕斌). CIESC Journal(化工学报), 2013, 64(10): 3493.

[7] YIN Yan-xia, XIANG Ben-qiong, TONG Li(尹燕霞, 向本琼, 佟丽). Experimental Technology and Management(实验技术与管理), 2010, 27(2): 33.

[8] SUN Yang, FAN Jun, HU Xiao-yun, et al(孙洋, 樊君, 胡晓云, 等). Acta Chimica Sinica(化学学报), 2011, 69(8): 937.

[9] XUAN Guang-shan, WU Xiao-nan, LI Yu-ping(玄光善, 吴效楠, 李玉平). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2005, 22(4): 861.

[10] Zhang J, Yan Q, Liu J, et al. Journal of Luminescence, 2013, (134): 747.

[11] Abou-Zied O K, Al-Shihi O I K. J. Am. Chem. Soc., 2008, 130(32): 10793.

[12] HU Meng-yao, PENG Mao, WU Hui, et al(胡梦瑶, 彭毛, 吴辉, 等). Chinese Journal of Colloid and Polymer(胶体与聚合物), 2009, 27(3): 33.

[13] Cui F C, Fan J, Li J L, et al. Bioorganic and Medicinal Chemistry, 2004, 12(1): 151.

[14] Larissa A, Rodrigo C, Betina G Z, et al. Materials Science and Engineering, 2009, C(29): 524.

[15] Ge Feng, Jiang Lixiang, Liu Diqiu, et al. Analytical Sciences, 2011, 27(1): 73.

[16] Ross P R, Subramanian S. Biochemistry, 1981, 20(11): 9102.

[17] Luo Y, Zhang B, Whent M, et al. Colloids Surf. B, 2011, 85(2): 145.

[18] Baptista M S, Indig G L. J. Phys. Chem. B, 1998, 102(23): 4678.

[19] ZHOU Neng, PAN Tong, LIANG Yi-zeng(周能, 潘彤, 梁逸曾). Lishizhen Medicine and Materia Medica Research(时珍国医国药), 2009, 20(12): 2964.

[20] HE Li-fang, LIN Dan-li, LI Yao-qun(何立芳, 林丹丽, 李耀群). Progress in Chemistry(化学进展), 2004, 16(6): 879.

管骁, 刘静, 殷婷, 李景军, 廖丽丽. 复合纳米颗粒中白藜芦醇-大麦醇溶蛋白相互作用研究[J]. 光谱学与光谱分析, 2016, 36(1): 163. GUAN Xiao, LIU Jing, YIN Ting, LI Jing-jun, LIAO Li-li. Study on the Interaction in Resveratrol-Hordein Nanoparticle[J]. Spectroscopy and Spectral Analysis, 2016, 36(1): 163.

复合纳米颗粒中白藜芦醇-大麦醇溶蛋白相互作用研究

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