Confocal imaging of Bim translocation to endoplasmic reticulum during DHA-induced ASTC-a-1 cell apoptosis

Min Chen; Yingying Lu; Tongsheng Chen; Junle Qu

Chinese Optics Letters, 2010, 8 (10): 950, Published Online: Oct. 19, 2010

Confocal imaging of Bim translocation to endoplasmic reticulum during DHA-induced ASTC-a-1 cell apoptosis Download： 802次

论文大纲

Min Chen ¹Yingying Lu ¹Tongsheng Chen ¹Junle Qu ²

Author Affiliations

¹ MOE Key Laboratory of Laser Life Science &

² Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China

共聚焦成像 Bim 内质网双氢青蒿素 170.1530 Cell analysis 170.1790 Confocal microscopy 170.2655 Functional monitoring and imaging

Abstract

Bim, a proapoptotic member of Bcl-2 family, plays an important role in cell apoptosis. It is generally thought that Bim translocates to mitochondria in response to apoptotic stimuli. We use confocal microscopy to image the temporal-spatial dynamics of Bim during dihydroartemisinin (DHA) induced human lung adenocarcinoma (ASTC-a-1) cell apoptosis. Interestingly, we find that DHA induces Bim translocation to endoplasmic reticulum (ER) rather than mitochondria, implying that Bim-ER pathway might be involved in the DHA-induced ASTC-a-1 cell apoptosis.

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Min Chen, Yingying Lu, Tongsheng Chen, Junle Qu. Confocal imaging of Bim translocation to endoplasmic reticulum during DHA-induced ASTC-a-1 cell apoptosis[J]. Chinese Optics Letters, 2010, 8(10): 950.

Confocal imaging of Bim translocation to endoplasmic reticulum during DHA-induced ASTC-a-1 cell apoptosis Download： 802次

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