幼年到成年斑马鱼大脑的OCT活体三维可视化

毛广娟; 林燕萍; 陈婷如; 张艺晴; 邱婷; 蓝银涛; 向湘; 傅洪波; 张建

doi:doi:10.3788/CJL202047.1207002

中国激光, 2020, 47 (12): 1207002, 网络出版: 2020-11-17

幼年到成年斑马鱼大脑的OCT活体三维可视化下载： 1336次

OCT in vivo Three-Dimensional Visualization of Zebrafish Brains from Juvenile to Adult

毛广娟 ¹林燕萍 ¹陈婷如 ¹张艺晴 ¹邱婷 ¹蓝银涛 ¹向湘 ¹傅洪波 ¹张建 ^1,2,*

作者单位

¹ 广州医科大学基础医学院生物医学工程系, 广东广州 511436

² 广州医科大学国家呼吸系统疾病临床医学研究中心呼吸疾病国家重点实验室, 广东广州 510120

图 & 表

图 1. OCT系统和斑马鱼样品。(a)OCT装置图以及凝胶固定的斑马鱼照片;(b)三个年龄段的斑马鱼样品照片

Fig. 1. OCT system and zebrafish samples. (a) Schematic of OCT setup and photograph of zebrafish hold by agar; (b) photograph of zebrafish samples from three age groups

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图 2. 斑马鱼头部的OCT活体成像。(a)连续的B扫描OCT图像;(b)重建的三维OCT图像;(c)三维OCT图像的虚拟分割结果

Fig. 2. OCT in vivo imaging of zebrafish head. (a) Continuous B-scan OCT images; (b) constructed three-dimensional OCT images; (c) virtual segmentation results of three-dimensional OCT images

下载图片查看原文

图 3. 三个年龄段斑马鱼大脑的冠状面图像。(a) OCT图;(b)切片染色图

Fig. 3. Coronal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

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图 4. 三个年龄段斑马鱼大脑的水平面图像。(a) OCT图;(b)切片染色图

Fig. 4. Horizontal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

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图 5. 测量斑马鱼大脑的体积。(a)斑马鱼大脑的三维图;(b)大脑体积测量结果

Fig. 5. Measured brain volume of zebrafish. (a) Three dimensional images of zebrafish brains; (b) measured results of brain volume

下载图片查看原文

图 6. 基于图4得到的斑马鱼大脑的面积。(a)~(c)切片染色结果和OCT结果的对比;(d)~(f)放大的切片染色结果

Fig. 6. Brain area of zebrafish obtained based on Fig. 4. (a)--(c) Comparison of sectional staining results and OCT results; (d)--(f) enlarged sectional staining results

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表 1斑马鱼活体成像技术的对比^{[8, 11, 25-28]}

Table1. Comparison of in vivo imaging techniques of zebrafish ^{[8, 11, 25-Imagingtechnique Contrast source Resolution Penetrationdepth Imagingobject Imagingspeed Label Cost
Confocalmicroscopy Fluorescence <1μm <500μm Embryo Low Yes *
Light sheetmicroscopy Fluorescence 1--10μm 0.1--5mm Embryo andinfant Fast Yes *
Photoacoustictomography Opticalabsorption 100μm--1mm <10mm Adult Fast No *
Micro-CT X-ray attenuation 2--3mm No limited Adult Medium No *
MRI H⁺ concentration 2--3mm No limited Adult Low No **
OCT Optical scattering 1--10μm 2--3mm Infant and adult Fast No
查看原文}

毛广娟, 林燕萍, 陈婷如, 张艺晴, 邱婷, 蓝银涛, 向湘, 傅洪波, 张建. 幼年到成年斑马鱼大脑的OCT活体三维可视化[J]. 中国激光, 2020, 47(12): 1207002. Mao Guangjuan, Lin Yanping, Chen Tingru, Zhang Yiqing, Qiu Ting, Lan Yintao, Xiang Xiang, Fu Hongbo, Zhang Jian. OCT in vivo Three-Dimensional Visualization of Zebrafish Brains from Juvenile to Adult[J]. Chinese Journal of Lasers, 2020, 47(12): 1207002.

幼年到成年斑马鱼大脑的OCT活体三维可视化下载： 1336次

图 1. OCT系统和斑马鱼样品。(a)OCT装置图以及凝胶固定的斑马鱼照片;(b)三个年龄段的斑马鱼样品照片

Fig. 1. OCT system and zebrafish samples. (a) Schematic of OCT setup and photograph of zebrafish hold by agar; (b) photograph of zebrafish samples from three age groups

图 2. 斑马鱼头部的OCT活体成像。(a)连续的B扫描OCT图像;(b)重建的三维OCT图像;(c)三维OCT图像的虚拟分割结果

Fig. 2. OCT in vivo imaging of zebrafish head. (a) Continuous B-scan OCT images; (b) constructed three-dimensional OCT images; (c) virtual segmentation results of three-dimensional OCT images

图 3. 三个年龄段斑马鱼大脑的冠状面图像。(a) OCT图;(b)切片染色图

Fig. 3. Coronal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

图 4. 三个年龄段斑马鱼大脑的水平面图像。(a) OCT图;(b)切片染色图

Fig. 4. Horizontal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

图 5. 测量斑马鱼大脑的体积。(a)斑马鱼大脑的三维图;(b)大脑体积测量结果

Fig. 5. Measured brain volume of zebrafish. (a) Three dimensional images of zebrafish brains; (b) measured results of brain volume

图 6. 基于图4得到的斑马鱼大脑的面积。(a)~(c)切片染色结果和OCT结果的对比;(d)~(f)放大的切片染色结果

Fig. 6. Brain area of zebrafish obtained based on Fig. 4. (a)--(c) Comparison of sectional staining results and OCT results; (d)--(f) enlarged sectional staining results

表 1斑马鱼活体成像技术的对比^{[8, 11, 25-28]}

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图 1. OCT系统和斑马鱼样品。(a)OCT装置图以及凝胶固定的斑马鱼照片;(b)三个年龄段的斑马鱼样品照片

Fig. 1. OCT system and zebrafish samples. (a) Schematic of OCT setup and photograph of zebrafish hold by agar; (b) photograph of zebrafish samples from three age groups

图 2. 斑马鱼头部的OCT活体成像。(a)连续的B扫描OCT图像;(b)重建的三维OCT图像;(c)三维OCT图像的虚拟分割结果

Fig. 2. OCT in vivo imaging of zebrafish head. (a) Continuous B-scan OCT images; (b) constructed three-dimensional OCT images; (c) virtual segmentation results of three-dimensional OCT images

图 3. 三个年龄段斑马鱼大脑的冠状面图像。(a) OCT图;(b)切片染色图

Fig. 3. Coronal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

图 4. 三个年龄段斑马鱼大脑的水平面图像。(a) OCT图;(b)切片染色图

Fig. 4. Horizontal views of images of zebrafish brains from three age groups. (a) OCT images; (b) sectional staining images

图 5. 测量斑马鱼大脑的体积。(a)斑马鱼大脑的三维图;(b)大脑体积测量结果

Fig. 5. Measured brain volume of zebrafish. (a) Three dimensional images of zebrafish brains; (b) measured results of brain volume

图 6. 基于图4得到的斑马鱼大脑的面积。(a)~(c)切片染色结果和OCT结果的对比;(d)~(f)放大的切片染色结果

Fig. 6. Brain area of zebrafish obtained based on Fig. 4. (a)--(c) Comparison of sectional staining results and OCT results; (d)--(f) enlarged sectional staining results

表 1斑马鱼活体成像技术的对比[8, 11, 25-28]

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幼年到成年斑马鱼大脑的OCT活体三维可视化下载： 1336次

表 1斑马鱼活体成像技术的对比^{[8, 11, 25-28]}