1 清华大学深圳国际研究生院, 广东省偏振光学检测与成像工程技术研究中心,深圳市无损监测与微创医学技术重点实验室, 光学检测与成像研究所, 广东 深圳 518055
2 清华大学生物医学工程系, 北京 100084
3 清华大学清华-伯克利深圳学院, 广东 深圳 518071
4 深圳市第六人民医院(南山医院), 华中科技大学协和深圳医院, 广东 深圳 518052
5 清华大学物理系, 北京 100084
近年来,随着新型光学器件的出现与数据处理能力的提升,偏振光学成像技术在生物医学领域的应用逐渐增多。穆勒矩阵因可以完备地表述样品的偏振信息,且测量装置可与传统光学仪器兼容,非常适合于生物医学样品的研究。同时,与非偏振光学方法相比,穆勒矩阵对亚波长结构敏感,还可以额外提供包括双折射、二向色性在内的样品的光学各向异性信息。介绍了穆勒矩阵偏振成像的基本理论与装置,包括穆勒矩阵参量的提取、背向散射成像、偏振显微成像、偏振内窥与偏振染色等在生物医学检测中具有一定应用潜力的方法和技术,并展示了穆勒矩阵成像在肝癌、乳腺癌、胃肠肿瘤等多种病理组织辅助诊断中的最新研究进展。穆勒矩阵偏振成像作为一种非标记、定量化、无损伤的快速检测技术,在生物医学领域显示出了广阔的应用前景。
医用光学 穆勒矩阵 偏振测定 散射 微观结构 生物医学
Author Affiliations
Abstract
1 Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
2 Department of Dermatology, The Sixth People’s Hospital of Shenzhen, Shenzhen 518052, China
3 Department of Pathology, The Sixth People’s Hospital of Shenzhen, Shenzhen 518052, China
4 Bioimaging Core, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR China
The aim of this study is to develop a novel technique for improving the intraoperative margin assessment of glioblastoma by examining the total extrinsic extracellular matrix (ECM) with eosin staining using fluorescence lifetime imaging microscopy (FLIM) and scale-invariant feature transform (SIFT) descriptor analysis. Pseudo-color FLIM images obviously exhibit ECM distributions, changes in sequential sections, and different regions of interest. Meanwhile, SIFT descriptors are first utilized for the discrimination of glioblastoma margins by matching similar ECM regions and extracting keypoint orientations from FLIM images obtained from a series of continuous slices. The findings indicate that FLIM imaging with SIFT analysis of the total ECM is a promising method for improving intraoperative diagnosis of frozen and surgically excised brain specimen sections.
170.3650 Lifetime-based sensing 170.3880 Medical and biological imaging 170.4580 Optical diagnostics for medicine Chinese Optics Letters
2017, 15(9): 090006
