1 辽宁石油化工大学化学化工与环境学部, 辽宁 抚顺 113006
2 河南省新能源电池材料工程技术研究中心, 河南省先进电池材料开发应用研究中心, 河南生物分子识别与传感重点实验室, 商丘师范学院化学化工学院, 河南 商丘 476000
3 郑州大学化学与分子工程学院, 河南 郑州 450001
H2O2是一种常见的生物活性小分子, 其在细胞增殖, 炎症反应, 细胞信号传输等过程中都起着重要的作用。 细胞粘度是一项重要的生理微环境参数, 其为细胞是否正常运转的关键指标。 研究还表明, 一些重大的生理疾病与细胞组织的粘度及过氧化氢浓度异常均有着密切的关联, 如阿尔茨海默病、 癌症。 因此开发能有效同时检测H2O2及粘度的分析方法对揭示相关生理病理机制及重大疾病的诊断有着重要的意义。 研制了一种能有效检测H2O2与粘度的双响应型荧光探针(1)。 由于存在扭曲分子内电荷转移(TICT)的猝灭效应, 探针(1)几乎不具有荧光发射特性。 而随着环境体系粘度的增高, TICT效应被抑制, 探针(1)呈现出了强的荧光发射, 且发射峰位于近红外波段处(680 nm)。 随着粘度从1.996 cp增至851.8 cp, 探针的荧光强度增幅达到了85倍。 探针还能对H2O2产生灵敏的荧光响应, 发射峰位置在590 nm处。 响应原理为H2O2能与探针中的苯硼酸基团反应使探针脱除掉与吡啶氮原子相连的亚甲基苯硼酸, 从而减弱了探针分子中的TICT效应以及分子内电荷转移(ICT)效应, 因此探针溶液荧光发射显著增强且探针溶液的紫外光谱发生了明显的蓝移(吸收峰从540 nm移至460 nm), 对应溶液颜色从红色变为黄色。 荧光光谱测试表明探针(1)对H2O2的检测具有高的选择性与灵敏度, 探针体系在590 nm处的荧光强度与H2O2的浓度呈良好的线性关系, 线性范围为0～25 μmol·L-1, 根据IUPAC的3σ法计算得出检测限为0.34 μmol·L-1。 此外细胞荧光成像实验表明探针(1)具有较好的生物相容性及细胞膜通透性, 且能实现细胞内H2O2的成像分析。
荧光探针 过氧化氢 粘度 近红外 苯并噻唑 Fluorescent probe Hydrogen peroxide Viscosity Near-infrared Benzothiazole 光谱学与光谱分析
2020, 40(6): 1775
Author Affiliations
Abstract
1 Center for Photonics and Electronics, Department of Precision Instrument, Tsinghua University, Beijing 100084, China
2 Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
A 21.2 kW, 1.94 times the diffraction-limit quasi-continuous-wave laser is presented in this Letter based on a multi-stage, power-scalable Yb:YAG master-oscillator-power-amplifier (MOPA) system under adaptive optics (AO) control. The output laser of the MOPA system is a rectangular beam with a length-width ratio of 2:1, a 200 μs pulse duration, and a 1000 Hz repetition rate. With the AO control system, the beam quality of the laser is improved from 4.20 to 1.94 times the diffraction limit. To our knowledge, this is the best quality laser in the 20 kW class except for combined lasers.
diode-pumped lasers ytterbium lasers adaptive optics Chinese Optics Letters
2020, 18(6): 061402
Author Affiliations
Abstract
1 Center for Photonics and Electronics, Department of Precision Instruments, Tsinghua University, Beijing 100084, China
2 Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
In this Letter, a 116-actuator deformable mirror (DM) was used to correct the wavefront distortion in a 10.7 J, 10 Hz neodymium-doped yttrium aluminum garnet (Nd:YAG) slab amplifier. By applying a pump-light homogenizer to transform the 3 × 1 near-field beam array into an integrated beam, the beam quality was greatly improved from 5.54 times diffraction limit (TDL) to 1.57 TDL after being corrected by the DM. To the best of our knowledge, this is the first investigation on beam quality control of an arrayed near-field beam in high-energy diode-pumped solid-state lasers.
140.3280 Laser amplifiers 140.3480 Lasers, diode-pumped 220.1080 Active or adoptive optics 220.1000 Aberration compensation Chinese Optics Letters
2019, 17(5): 051403
