针对目前常用的便携式眼动仪存在易受外界环境以及使用者生理和心理状态变化等因素影响的问题,提出一种可用于眼动跟踪的紧凑型巩膜血管成像装置。该成像装置由一块主透镜、一块微透镜阵列以及一个成像探测器组成,其中主透镜的焦距等于眼球表面到主透镜的距离,成像探测器位于微透镜阵列的1倍焦距处。为了进一步减小整个成像装置的体积,采用直角棱镜将光路进行两次转折,按照光路顺序依次是成像物体、主透镜、直角棱镜、反射面 1、反射面 2、微透镜阵列和成像探测器。结果表明,该装置基于微透镜阵列实现巩膜血管的近距离清晰成像,能够获得巩膜血管的多孔径图像阵列,具有体积小、景深大和分辨力高的特点。

Multiphoton microscopy (MPM), with the advantages of improved penetration depth, decreased photo-damage, and optical sectioning capability, has become an indispensable tool for biomedical imaging. The combination of multiphoton fluorescence (MF) and second-harmonic generation (SHG) microscopy is particularly effective in imaging tissue structures of the ocular surface. This work is intended to be a review of advances that MPM has made in ophthalmic imaging. The MPM not only can be used for the label-free imaging of ocular structures, it can also be applied for investigating the morphological alterations in corneal pathologies, such as keratoconus, infected keratitis, and corneal scar. Furthermore, the corneal wound healing process after refractive surgical procedures such as conductive keratoplasty (CK) can also be studied with MPM. Finally, qualitative and quantitative SHG microscopy is effective for characterizing corneal thermal denaturation. With additional development, multiphoton imaging has the potential to be developed into an effective imaging technique for in vivo studies and clinical diagnosis in ophthalmology.

为探索飞秒激光在兔眼巩膜上产生光离解作用的可行性,并寻找适当的激光切割方式及相关参数,将不同脉冲能量的飞秒激光(800 nm/50 fs)聚焦后作用于离体兔眼巩膜。通过计算机控制的三维平台的定向移动,飞秒激光能够在兔眼巩膜上完成打孔、蛇形扫描和线性切割三种方式的光离解作用。应用光学显微镜和扫描电镜(SEM)观察激光作用后巩膜的形态学变化,并用Nd:YAG激光作为对比。实验结果表明,飞秒激光经过显微物镜(NA：0.2)聚焦后,当其功率密度达到或超过9.55×1014 W/cm2,脉冲能量在37.5～125 μJ变化时,激光以0.1 mm/s的速度线性扫描巩膜能形成深度为30～70 μm的沟道; 当激光的功率密度减小至7.96×1014 W/cm2,脉冲能量小于31.25 μJ/pulse时,在相同条件下却不能产生光离解作用。与Nd:YAG激光相比,飞秒激光在兔眼巩膜上切割瘘道的内壁更加光滑整齐,对周围的组织损伤更小。飞秒激光对离体兔眼巩膜高精度和微创伤的光离解作用,预示了它在未来青光眼治疗中有潜在的应用价值。