基于双输出皮秒脉冲激光器和激光扫描显微镜平台，构建了一套可实现双光子和多种四波混频（FWM）效应的多模式成像系统，可实现光谱采集和良好的成像功能。等离激元材料可以提高FWM信号，在FWM成像下具有超高的成像对比度。以5 nm金纳米粒子作为成像探针，利用等离激元增强FWM成像追踪了其在小鼠肝脏和肾脏组织内的代谢残留。该研究为体内蛋白类药物的代谢评价应用提供可能。

In this study, several imaging functions, such as two-photon imaging and multi-mode four wave mixing(FWM) imaging, were realized on a single multiphoton spectrometer by the combination of double-output picosecond pulse laser and laser scanning microscope platform. Different nonlinear effects were tested to prove that this system could achieve spectroscopic recording and have excellent imaging performance. We explored the possible application of plasmon-enhanced FWM imaging for the metabolism residues of antibody drugs in a mouse's liver and kidney using 5 nm gold nanoparticles as a probe. This method acts as a tool for conducting the metabolism evaluation of protein drugs in the body.