散射介质内目标探测和成像技术在生物医学领域具有广泛应用，但目前的探测方法仍存在外部激发光的使用效率低或者聚焦位置不确定等问题。为此，提出一种利用区域方差反馈的波前整形技术实现散射介质内非侵入式深度可选择聚焦。该技术利用目标散斑的区域方差作为波前整形的反馈信号，通过空间光调制器对输入波前进行相位调制，使散射介质内的激发散斑聚焦到单个目标点上。结果表明，区域方差的波前整形算法不仅能够实现散射介质内目标的深度聚焦，还可根据区域选择将外部输入激光聚焦在确定的目标位置上。这种可选择聚焦技术为生物组织内多目标成像提供一种新的技术途径。

Detection and imaging technology in a scattering medium is widely used in the biomedical field. However, the current technology still has some problems, such as low efficiency of external excitation or uncertain focus position. Thus, this paper proposed region variance feedback using the wavefront shaping method to realize non-invasive focusing deep in a scattering medium. Here, the region variance of the target speckle was used as the feedback of the wavefront shaping, and the excitation speckle in the scattering medium was focused on a single target by phase modulating with a spatial light modulator. The results show that the region variance method using the wavefront shaping realizes deep focusing on a single target in the scattering medium and selectively focuses on the determined target position according to the region selection. This selective focusing technology provides a new way for multiple targets imaging in biological tissue.