无创且实时检测微观环境的pH变化在医疗等领域具有重要的研究价值, 本文通过灵活简单的共沉淀方法制备了一种基于pH敏感分子异硫氰酸荧光素(FITC)的荧光pH纳米传感器, FITC的荧光强度随pH值增加发生明显变化, 当pH值从3变至9时, 荧光强度增大约38倍, 基于FITC荧光强度变化可实现对pH的灵敏检测, pKa值为 6.07。该荧光pH纳米传感器具有小粒径、高灵敏度、良好的可逆性和生物相容性, 在细胞等微环境pH检测方面将具有良好的应用前景。

The non-invasive and real-time pH detection is of significant importance in biomedicine fields. In this paper, a fluorescent pH nanosensor based on flourescein isothiocyanate(FITC) is prepared by a facile coprecipiation method. The fluorescence intensity of FITC changes obviously with the increase of pH. When the pH changes from 3 to 9, the fluorescence intensity of FITC increases up to about 38 times. The pH detection can be realized by measuring fluorescence intensity changes of FITC. The value of pKa is calculated to be 6.07. The fluorescent pH nanosensor has small particle size, high sensitivity, good reversibility and excellent biocompatibility, which is promising in intracellular pH detection.