为探讨在体皮肤实验中最为简便有效的给药方式, 将化学渗透促进剂噻酮与聚乙二醇400（PEG400）的混合溶液直接作用于去除部分角质和含角质的在体大鼠皮肤, 利用CCD拍照进行直观观察, 并通过反射光谱的变化来反映皮肤光透明效果, 同时与单纯PEG400作用进行了对比。结果表明：混合溶液可以使去除部分角质皮肤在15 min内反射光谱明显下降, 并产生显著的光透明效果, 30 min后皮肤更加透明; 而单纯PEG400以及未去除角质层的完整皮肤均无光透明效果。因此, 结合物理方法去除部分角质层、并添加促渗剂噻酮, 能以非侵入方式, 快速、有效提高PEG400对在体皮肤的光透明效果。

In quest of the most simple and effective treatment for in vivo skin experiments, thiazone was used as an enhancer in our study. To compare with the pure polyethyleneglycol 400 (PEG400) solution, the mixed solution (thiazone in combination with) was applied on the stratum corneum partly removed skin and intact skin respectively. For visual observation, photographs of the local skins were taken by CCD; and then the light reflectance spectra of skin were monitored. The results showed that a mixed solution could make the skin without stratum corneum more transparent in 15 min, with the reflectance spectrum decreased significantly; while there’s no observable change in the intact skins. However, the pure PEG400 did not have optical clearing effects on any skins. In conclusion, the non-invasive drug delivery method, which is combined with physically abrading part of the stratum corneum and adding chemical enhancer-thiazone, can improve the effect of PEG400 in skin optical clearing quickly and effectively.