光学学报, 2017, 37 (3): 0318005, 网络出版: 2017-03-08
随机光学重构显微成像技术及其应用 下载: 2251次
Stochastic Optical Reconstruction Microscopy Imaging Technique and Application
显微 超分辨成像 随机光学重构显微镜 分子生物学 细胞生物学 microscopy super-resolution imaging stochastic optical reconstruction microscopy molecular biology cell biology
摘要
光学显微成像技术在生命科学、生物医学、临床医学诊断和材料科学等领域有着非常广泛的应用。但由于光学衍射极限的存在, 传统光学显微镜无法观察到纳米尺度的物质及生命活动, 极大地限制科学研究和医学的发展。近年来, 随着突破光学衍射极限的超分辨成像技术的不断发展, 显微成像分辨率得到不同程度的提高。目前在基于不同原理的各种超高分辨率显微镜中, 随机光学重构显微镜 (STORM) 分辨率最高, 可达几十纳米, 真正实现了单分子水平检测。着重介绍了STORM超分辨显微成像技术的原理、实验方法及其应用。
Abstract
Optical microscope is widely used in many fields such as life science, biomedicine, clinical diagnosis and material science. But due to the optical diffraction limit, the traditional optical microscope cannot reach the nanoscale resolution, which greatly restricts the development of science and medicine. In recent years, a number of super-resolution fluorescence microscopy techniques are invented to overcome the diffraction barrier and the imaging resolution are improved at various degrees. Among various super high resolution microscopes based on different principles, the stochastic optical reconstruction microscopy (STORM) reaches the highest spatial resolution, about tens of nanometer, and therefore it can realize the single molecule detection. The principle, experimental method and its application of STORM super resolution microscope are mainly introduced.
杨洁, 田翠萍, 钟桂生. 随机光学重构显微成像技术及其应用[J]. 光学学报, 2017, 37(3): 0318005. Yang Jie, Tian Cuiping, Zhong Guisheng. Stochastic Optical Reconstruction Microscopy Imaging Technique and Application[J]. Acta Optica Sinica, 2017, 37(3): 0318005.