中国激光, 2018, 45 (2): 0207028, 网络出版: 2018-02-28
基于静电富集-表面增强拉曼光谱联用技术的抗生素检测 下载: 990次
Detection of Antibiotics Based on Hyphenated Technique of Electrostatic-Preconcentration and Surface-Enhanced-Raman-Spectroscopy
医用光学 抗生素检测 表面增强拉曼散射 静电富集 灵敏度 银纳米颗粒 medical optics antibiotic detection surface-enhanced Raman scattering electrostatic preconcentration sensitivity Ag nanoparticles
摘要
利用表面增强拉曼光谱(SERS)和静电富集(EP)相结合的技术,实现了水环境中磺胺甲基嘧啶、丁胺卡那霉素、恩诺沙星和环丙沙星的有效富集和快速痕量探测。实验结果表明,与非静电富集SERS探测相比较,磺胺甲基嘧啶和丁胺卡那霉素的特征峰强度提高了大约10倍,恩诺沙星和环丙沙星的特征峰强度提高了2~3倍;4种抗生素的最低检测浓度分别为1.9×10-8,1.7×10-8,5.5×10-8 ,6.0×10-8 mol·L-1;当被检测目标的浓度较低时,特征峰强度与探测浓度具有良好的线性关系。EP-SERS技术可有效提高水环境中抗生素的检测灵敏度。
Abstract
The effective accumulation and the rapid trace detection of sulfadiazine, amikacin, enrofloxacin and ciprofloxacin are realized by the hyphenated technology of surface enhanced Raman spectroscopy (SERS) and electrostatic preconcentration (EP). The experimental results show that, if compared with those by the non-EP-SERS detection, the characteristic peak intensities of sulfadiazine and amikacin are increased by about 10 times, and the characteristic peak intensities of enrofloxacin and ciprofloxacin are increased by 2-3 times. The minimum detectable concentrations of the 4 kinds of antibiotics are 1.9×10-8, 1.7×10-8, 5.5×10-8 and 6.0×10-8 mol·L-1, respectively. A good linear relationship between the characteristic peak intensity and detection concentration appears when the concentration of detected targets is relatively low. The detection sensitivity of antibiotics in aqueous can be improved effectively by using the EP-SERS technology.
马海宽, 张旭, 钟石磊, 史晓凤, 马丽珍, 马君. 基于静电富集-表面增强拉曼光谱联用技术的抗生素检测[J]. 中国激光, 2018, 45(2): 0207028. Ma Haikuan, Zhang Xu, Zhong Shilei, Shi Xiaofeng, Ma Lizhen, Ma Jun. Detection of Antibiotics Based on Hyphenated Technique of Electrostatic-Preconcentration and Surface-Enhanced-Raman-Spectroscopy[J]. Chinese Journal of Lasers, 2018, 45(2): 0207028.