生物样品存在着丰富的物质信息，每一种物质都有其自身独特的性质及功能，对这些物质在生物体内的产生、转运、分布等进行研究可以使我们更加深入地了解生物系统。具备良好化学特异性的光学显微镜为这些研究提供了强大且可靠的工具。拉曼散射技术由于解决了荧光显微镜所需的外源性标记所带来的问题，极大地促进了生物医学的发展。高光谱相干拉曼散射（hyperspectral coherent Raman scattering，HS-CRS）技术可对不同成分物质进行无标记化学成像，通过同时提供图像和光谱信息，对样品内物质的性质、含量和分布状况做出判断，为科研人员提供了更多维度的物质信息。HS-CRS技术在近几十年内进行了不断的技术更新并在生物医学领域完成新的技术应用，本文对主要的HS-CRS技术的实现形式及方案进行了介绍，并总结了HS-CRS技术在生物医学领域的应用。

There is abundant material information in biological samples, and each material has its unique properties and functions. Research on the production, transportation, and distribution of these materials in organisms can give us a deep understanding of biological systems. Optical microscopes with chemical specificity are powerful and reliable tools for these studies. Raman scattering technology has greatly promoted the development of biomedicine because it solves the problems caused by the fluorescence microscope. Especially, hyperspectral coherent Raman scattering (HS-CRS) microscope can perform label-free chemical imaging of substances with different components. By providing image and spectral information at the same time, the properties, content and distribution of substances in the sample can be determined. It provides scientific researchers with more dimensional material information. In recent decades, HS-CRS has been continuously updated and applied in the biomedical field. This paper introduces the main HS-CRS implementation and schemes and summarizes its application in the biomedical field.