地表水微囊藻毒素的表面等离波子共振免疫检测方法研究

结合表面等离波子共振(SPR)技术与免疫检测技术，研究和建立了一种响应速度快、免标记、低成本的地表水微囊藻毒素（MC-LR）检测方法。基于SpreetaTM传感器构建了小型SPR免疫检测系统，采用共价偶联方法在传感器金膜表面修饰MC-LR-BSA抗原为生物敏感膜；开展了MC-LR的SPR免疫检测方法实验研究，结果表明该方法的相对标准偏差为1.0%(n=6)，定量范围为2~32 ng/mL，检测限为0.63 ng/mL，半抑制浓度CI50为10.7 ng/mL，空白加标回收率和样品加标回收率在90%~113%之间。实样检测实验中，管道末梢饮用自来水水样未能检测出MC-LR，而某湖水水样中MC-LR的质量浓度为2.46 ng/mL。实验研究与结果表明，MC-LR的SPR免疫检测方法可以满足世界卫生组织(WHO)对于饮用水中MC-LR最低含量检测的需求。

Abstract

A novel assay detection method for microcystin-LR (MC-LR) in surface water is researched and established which combines surface plasmon resonance (SPR) technique with immunoassay method. SpreetaTM sensor is adopted in the SPR flow injection analysis (FIA) system. The reusable biosensor chip is functionalized with (MC-LR)-BSA using covalent-coupling method. Experimental studies of SPR immunoassay method for MC-LR are carried out, the results show that relative standard deviation (RSD), quantitative range, detective limit and half maximal inhibitory concentration (CI50) are 1.0% (n=6), 2~32 ng/mL, 0.63 ng/mL and 10.7 ng/mL,respectively. Recovery rate of blank sample and different water samples spiked MC-LR ranges from 90% to 113%. It is concluded that tap water does not contain MC-LR while pond water contains 2.46 ng/mL MC-LR in real sample assay. The experimental results demonstrate that the SPR immunoassay can well meet drinking water guidelines of the World Health Organization (WHO) for MC-LR.

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王晓萍, 詹舒越, 罗昭锋, 周宏敏. 地表水微囊藻毒素的表面等离波子共振免疫检测方法研究[J]. 光学学报, 2012, 32(2): 0212005. Wang Xiaoping, Zhan Shuyue, Luo Zhaofeng, Zhou Hongmin. Research of Surface Plasmon Resonance Immunoassay Method for Microcystin-LR in Surface Water[J]. Acta Optica Sinica, 2012, 32(2): 0212005.

地表水微囊藻毒素的表面等离波子共振免疫检测方法研究

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