N<sup>+</sup>离子注入诱变选育纤维素酶高产菌

陈阿娜; 汤斌; 刘标

doi:doi:10.3969/j.issn.1007-7146.2011.02.006

激光生物学报, 2011, 20 (2): 171, 网络出版: 2015-10-08

N⁺离子注入诱变选育纤维素酶高产菌

Screening of High-yield Cellulase-producing Strains by N⁺ Implantation Mutation

论文大纲

陈阿娜 ^*汤斌刘标

作者单位

安徽工程大学生物与化学工程学院, 安徽芜湖 241000

纤维素酶 N+离子注入传代培养发酵周期 cellulase N+ implantation subculture fermentation period

摘要

纤维素酶产生菌Rhizopus sp.TC1经过N+离子注入、紫外线、5-氟尿嘧啶(5-Fu)等诱变剂诱变, 选育得到突变株Rhizopus sp.TC1653, 发酵终点时滤纸酶活力(FPA)和羧甲基纤维素酶活力(CMC)分别为22.6 IU/g、224.7 IU/g干物质, 较出发菌株分别提高了276.7 ％和214.3 ％。突变株经传10代培养, 任意2代间酶活力差值均在10 %内, 表明该菌株产酶性能稳定, 可作为后继实验出发菌株。突变株在培养基上能够快速生长, 发酵96 h即达到酶活高峰期, 较绿色木霉酶活高峰期提前48 h, 缩短了发酵周期。该实验结果表明, 经N+离子注入等方式诱变选育的突变株具有孢子萌发率高, 同步性好, 代谢速度快等优点。

Abstract

Cellulase-producing strain Rhizopus sp.TC1 was subjected to mutation involving treatment of N+ implantation, UV followed by 5-Fu; successive mutant Rhizopus sp.TC1653 showed enhanced cellulase production. The FPA and CMC case of mutant TC1653 was 22.6 and 224.7 IU per gram of dry medium respectively at the end of fermentation, which was increased by 3.77 folds and 3.14 folds respectively compared to that of TC1. Ten times subculturing indicated that the mutant TC1653 had good genetic stability and character of cellulase production. TC1653 showed rapid growth on solid-state medium and its cellulase activities were highest at 96 hours. Fermentation time shortened to for 48 hours compared to that of Trichoderma virid. The results showed that mutant TC1653 had some advantages under the mutation caused by N+ implantation.

参考文献

[1] 中国生物工程学会. 中国生物技术产业发展报告［M］. 北京: 化学工业出版社, 2003: 79.

Chinese Society of Biotechnology. Development Report of China’s Biotechnology Industry［M］. Beijing: Chemical Industry Press, 2003: 79.

[2] LIU J, LIU M, WANG J, et al. Enhancement of the Gibberella zeae Growth Inhibitory Lipopeptides from a Bacillus subtilis Mutant by Ion Beam Implantation［J］. Appl Microbiol Biotechnol, 2005, 69: 223-228.

[3] 虞龙, 周剑, 余增亮. 低能离子注入L2乳酸生产菌种选育与发酵条件初步优化［J］. 激光生物学报, 2005, 14(3): 184-188.

YU Long, ZHOU Jian, YU Zeng-liang. Screening of L-lactic Acid Producing Bacteria by Low Energy Ions Implantation and Preliminary Research of Fermentation Conditions［J］. Acta Laser Biology Sinica, 2005, 14(3): 184-188.

[4] 李市场, 白爱枝, 姚建铭, 等. 低能离子注入木聚糖酶产生菌黑曲霉(Aspergillus niger)育种中参数的优化［J］. 激光生物学报, 2003, 12(5): 377-381.

LI Shi-chang, BAI Ai-zhi, YAO Jian-ming, et al. Optimization of Parameters in Breeding of Low-energy Ion Implantation in Xylanase-producing Strain Aspergillus niger［J］. Acta Laser Biology Sinica, 2003, 12(5): 377-381.

[5] 陈义光, 李铭刚, 徐丽华, 等. 新型物理诱变方法及其在微生物诱变育种中的应用进展［J］. 长江大学学报(自然科学版), 2005, 2(5): 46-50.

CHEN Yi-guang, LI Ming-gang, XU Li-hua, et al. New Approaches of Physical Mutagenesis and Its Application in Microorganism Breeding［J］. Journal of Jianghan Petroleum Institute(Social Science Edition), 2005, 2(5): 46-50.

[6] 沈萍, 范秀容, 李广武. 微生物学实验［M］. 北京: 高等教育出版社, 1981.

SHEN Ping, FAN Xiu-rong, LI Guang-wu. Experimental Microbiology［M］. Beijing: Higher Education Press, 1981.

[7] 陈书峰, 赵亮, 刘德华. 绿色木霉在稻壳和麸皮混合基质上固态发酵生产纤维素酶的研究［J］. 食品与发酵工业, 2004, 30(1): 9-12.

CHEN Shu-feng, ZHAO Liang, LIU De-hua. Solid-state Fermentation for Cellulase Production by Trichoderma viride 3.2942［J］. Food and Fermentation Industries, 2004, 30(1): 9-12.

[8] 陈阿娜, 汤斌. 一种改进的纤维素分解菌鉴别培养基［J］. 生物学杂志, 2006, 23(6): 48-49.

CHEN A-na, TANG Bin. An Improved Differential Medium for Cellulose Decomposing Microorganisms［J］. Journal of Biology, 2006, 23(6): 48-49.

[9] 陈阿娜, 汤斌, 张庆庆, 等. 根霉Rhizopus sp. TC1产酶条件的研究［J］. 安徽工程科技学院学报, 2006, 4(21): 20-22.

CHEN A-na, TANG Bin, ZHANG Qing-qing, et al. Fermentation Conditions for Cellulase Production by Rhizopus sp. TC1［J］. Journal of Anhui University of Technology and Science(Natural Science), 2006, 4(21): 20-22.

[10] MANDELS M, WEBER J. The Production of Cellulases［J］. Adv Chem Ser, 1969, 95: 391-414.

[11] 施巧琴, 吴松刚. 工业微生物育种学［M］. 北京: 科学出版社, 2003.

SHI Qiao-qing, WU Song-gang. Breeding of Industrial Microbiology［M］. Beijing: Science Press, 2003.

[12] 曾宪贤, 吕杰. 离子注入改良纤维分解菌及其机理研究［J］. 新疆大学学报(自然科学版), 2005, 22(1): 17-21.

ZENG Xian-xian, L Jie. Studies on Improvement and Mechanism of Bacteria of Cellulose Hydrolysis by Ion Implantation［J］. Journal of Xinjiang University (Natural Science Edition), 2005, 22(1): 17-21.

[13] ZHANG Qin, LO Chi-Ming, JU Lu-Kwang. Factors Affecting Foaming Behavior in Cellulase Fermentation by Trichoderma reesei Rut C-30［J］. Bioresource Technology, 2007, 98: 753-760.

[14] IKEDA Y, ENOCK Y P, OKUDA N. Bioconversion of Wwaste Office Paper to Gluconic Acid in a Turbine Blade Reactor by the Filamentous Fungus Aspergillus niger［J］. Bioresource Technology, 2006, 97: 1030-1035.

[15] SOLOV’EVA I V, OKUNEV O N, VEL’KOV V V, et al. The Selection and Properties of Penicillium verruculosum Mutants with Enhanced Production of Cellulases and Xylanases［J］. Microbiology, 2005, 74: 141-146.

陈阿娜, 汤斌, 刘标. N⁺离子注入诱变选育纤维素酶高产菌[J]. 激光生物学报, 2011, 20(2): 171. CHEN A-na, TANG Bin, LIU Biao. Screening of High-yield Cellulase-producing Strains by N⁺ Implantation Mutation[J]. Acta Laser Biology Sinica, 2011, 20(2): 171.

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