激光生物学报, 2018, 27 (1): 60, 网络出版: 2018-04-23  

水稻-油菜双序列复种免耕、翻耕土壤真菌多样性

Diversity of Filamentous Fungus Community in Paddy Fields with Different Tillage Methods
陈力力 1,2,3,*刘金 1,2,3李梦丹 2,3张立钊 2,3邹应斌 1,2黄敏 1,2
作者单位
1 湖南农业大学, 湖南 长沙 410128
2 南方粮油作物协同创新中心, 湖南 长沙 410128
3 食品科学与生物技术湖南省重点实验室, 湖南 长沙410128
摘要
研究水稻-油菜双序列复种免耕、翻耕稻田土壤真菌的多样性。采用MiSeq 高通量测序方法, 对湖南安乡(AX)、农大(ND)、南县(NX)3个相同处理试验点免耕、翻耕土壤样品进行ITS 区高通量测序、多样性比较分析、物种组成分析及样品聚类分析。结果表明6个样品共得到真菌优化序列221 105条, 分属于1 356个OTU, 真菌种类覆盖7门、121科、230属; 指数分析表明NT样品菌群相对丰度及群落多样性均略高于CT样品; 在门水平上各试验点菌群结构相似, 前三位为子囊菌门、接合菌门和担子菌门, 但是同一试验点NT样品子囊菌相对丰度均低于CT样品, 接合菌和担子菌相对丰度均高于CT样品。科属水平比较表明, 3个试验点NT样品被孢霉科相对丰度比CT样品的大3-5倍, 毛壳菌科的相对丰度小1-2倍; NT样品Carpoligna、栓菌属(Trametes)、网孢菌属 (Filobasidium)、被孢霉属(Mortierella)的序列数明显大于CT样品, 毛壳属(Chaetomium)、柄孢壳菌属(Podospora)及瓜亡革菌属(Thanatephorus)的序列数明显小于CT样品。土壤理化因子测定表明NT样品的有机质、全氮及速效钾含量均高于CT样品。本研究得出水稻-油菜双序列复种免耕、翻耕稻田土壤样品真菌物种组成丰富, 不同耕作方式土壤真菌种群结构存在差异, 菌群组成及丰度对土壤理化因子有一定影响。
Abstract
To study the diversity and community structure of filamentous fungus in rice-oilseed multiple cropping fields with convertional-tillage(CT) and no-tillages(NT). Micro-organism population analysis were studied based on ITS sequences analyzed by MiSeq. The results showed that 221 105 vaild sequences was obtained, it covered 1 356 OTU, 7 phylums, 121 families and 230 generaes; The index analysis showed that the relative abundance and community diversity of the NT samples were slightly higher than that of the CT samples. On the phylums level, the relative abundance of Ascomycota in NT samples was lower than that in CT and the zygotes and basidites was higher than that in CT; The vaild sequences of Carpoligna、Trametes、Filobasidium、Mortierella in NT samples was higher than that in CT and Chaetomium、Podospora、Thanatephorus was lower than that in CT on the generaes level. The physical and chemical factors of soil organic matter determination showed that the total nitrogen and available potassium content in NT samples were higher than those in CT samples. There are indifferences filamentous fungus population structure in rice-oilseed multiple cropping fields with convertional-tillage(CT) and rice-oilseed multiple cropping and no-tillages(NT).The composition and abundance of flora has a certain effect on the soil physical and chemical factors.
参考文献

[1] 谷子寒, 易镇邪, 周文新, 等. 土壤耕作方式对水稻产量形成特性的影响初探[J]. 作物研究, 2017, 31(2): 103-109.

    GU Zihan, YI Zhengxie, ZHOU Wenxin, et al. Preliminary study abotu the effects of soil tillage ways on the yield formation characteristics of rice[J]. Crop Resaerch, 2017, 31(2): 103-109.

[2] 成臣, 曾勇军, 杨秀霞, 等. 不同耕作方式对稻田净增温潜势和温室气体强度的影响[J]. 环境科学学报, 2015,(6): 1887-1895.

    CHENG Cheng, ZENG Yongjun, YANG Xiuxia, et al. Effect of different tillage methods on net global warming potential and greenhouse gas intensity in double ricecropping systems[J]. Acta Scientiae Circumstantiae, 2015, 35(6): 1887-1895.

[3] TIAN Shenzhong, WANG Yu, TANG Tangyuan, et al. Effect of tillage method changes on soil organic carbon pool in farmland under long-term rotary tillage and no tillage[J]. Transactions of the Chinese Society of Agricultural Engineering, 2016, 32(17): 98-105.

[4] FRIEDRICH T, DERPSCH R, KASSAM A. Overview of the global spread of conservation agriculture[J]. Field Actions Sci Rep, 2012, 6: 1941-1945.

[5] PITTELKOW C M, LIANG X, LINQUIST B A, et al. Productivity limits and potentials of the principles of conservation agriculture[J]. Nature, 2015, 517(7534): 365-368.

[6] 魏欢欢, 王仕稳, 杨文稼, 等.免耕及深松耕对黄土高原地区春玉米和冬小麦产量及水分利用效率影响的整合分析[J].中国农业科学, 2017, 50(3): 461-477.

    WEI Huanhuan, WANG Shiwen, YANG Wenjia, et al. Meta analysis on impact of no-tillage and subsoiling tillage on spring maize and winter wheat yield and water use efficiency on the loess plateau[J]. Scientia Agricultura Sinica, 2017, 50(3): 461-477.

[7] ENIO CAMPIGLIA, ROBERTO MANCINELLI, EMANUELE RADICETTI. Influence of no-tillage and organic mulching on tomato (Solanum lycopersicum L.) production and nitrogen use in the mediterranean environment of central Italy[J]. Scientia Horticulturae, 2011, 130(3): 588-598.

[8] 陈丹梅, 段玉琪, 杨宇虹, 等. 轮作模式对植烟土壤酶活性及真菌群落的影响[J]. 生态学报, 2016, 36(8): 2373-2381.

    CHEN Danmei, DUAN Yuqi, YANG Yuheng, et al. Influence of crop rotation on enzyme activities and fungal communities in flue-cured tobacco soil[J]. Acta Ecologica Sinica, 2016, 36(8): 2373- 2381.

[9] 王芳, 图力古尔. 土壤真菌多样性研究进展[J]. 菌物研究, 2014,(3): 178-186.

    WANG Fang, BAU Tolgor. Research advances in the diversity of soil fungi[J]. Journal of Fungal Research, 2014, (3): 178-186.

[10] SHARMA S K, RAMESH A, SHARMA M P, et al. Microbial community structure and diversity as indicators for evaluating soil quality[J]. Biodiversity, Biofuels, Agroforestry and Conservation Agriculture, Sustainable Agriculture Reviews, 2011, 5: 317-358.

[11] 谭悠久, 张荣, 张军林, 等. 中国毛壳菌科(Chaetomiaceae)分类研究[J]. 西北农林科技大学学报(自然科学版), 2005, 33(8): 267-269.

    TAN Youjiu, ZHANG Rong, ZHANG Junlin, et al. Classification of the family Chaetomiaceae from China[J]. Journal of Northwest Sci-Tech University of Agriculture and Forest, 2005, 33(8): 267-269.

[12] 何海清, 杨莉娜, 周金燕, 等. 紫外分光光度法和HPLC法测定角毛壳菌CH-1发酵液中卵孢菌素含量的比较研究[J]. 中国抗生素杂志, 2015, 40(8): 593-598.

    HE Haiqing, YANG Lina, ZHOU Jinyan, et al. Comparison of UV spectrophotometry and HPLC on quantitative determination of oosporein in the broth of Chaetomiun cupreum CH-1[J]. Chinese Journal of Antibiotics, 2015, 40(8): 593-598.

[13] 张海英, 夏宣宣. 连作土壤中施加球毛壳ND35菌肥对杨树生理特征与生物量的影响[J]. 天津农业科学, 2017,(9): 22-25, 34.

    ZHANG Haiying, XIA Xuanxuan. Effects of Chaetomium globosmn ND35 fungus fertilizer on physiological characteristics and biomass of poplar in replanted soil[J]. Tianjin Agricultural Sciences, 2017, (9): 22-25, 34.

[14] PUGET P, ANGERS D A, CHEN U C. Nature of carbohy-drates associated with water-stable aggregates of two cultivate soils[J]. Soil Biology & Biochemistry, 1999, 31(1): 55-63.

[15] 孟令男, 许修宏, 李洪涛, 等. 污泥堆肥对氯嘧磺隆残留及土壤中真菌群落结构的影响[J]. 农业环境科学学报, 2014, 33(3): 495-501.

    MENG Lingnan, XU Xiuhong, LI Hongtao, et al. Effects of sewage sludge compost on chlorimuron-ethyl residue and fungal community structure in soil[J].Journal of Agro-Environment Science, 2014, 33(3): 495-501.

[16] 张敏, 关随霞, 张玉先, 等. 响应面法优化深黄被孢霉产油突变株的发酵培养基配方[J]. 中国油脂, 2016,(3): 66-69.

    ZHANG Min, GUAN Suixia, ZHANG Yuxian, et al. Optimization of fermentation medium formula for the production of oil from Mortierella isabellina mutant using response surface methodology[J]. China Oils and Fats, 2016, (3): 66-69.

[17] ZHANG Xiaoqing, YUAN Jing, XIAO Yazhong, et al. A primary studies on molecular taxonomy of Trametes species based on the ITS sequences of rDNA[J]. Mycosystema, 2006, 25(1): 23-31.

[18] 胡渤洋, 王寿南, 陈青君, 等. 4种常见农药的耐受真菌筛选、鉴定及降解作用初探[J]. 北京农学院学报, 2017, 32(1): 7-14.

    HU Boyang, WANG Shounan, CHEN Qingjun, et al. Studies on screening, identification, and degrading activities of resistant gungi against four common pesticides[J]. Journal of Beijing University of Agriculture, 2017, 32(1): 7-14.

[19] 李新鑫, 余学军, 俞暾, 等. 竹林环境中木质素降解菌株的分离鉴定[J]. 竹子学报, 2016, 36(2): 20-25.

    LI Xinxing, YU Xuejun, YU Tun, et al. Separation and identification of lignin-degrading microorganisms from bamboo stand soils[J]. Journal of Bamboo Research, 2016, 36(2): 20-25.

[20] 王富乾, 蒋捷, 马浩然, 等. 一株毛壳属内生真菌次生代谢产物研究[J]. 中草药, 2017, 48(7): 1298-1301.

    WANG Fuqian, JIANG Jie, MA Haoran, et al. Study on secondary metabolites of endophytic Chaetomium sp.[J]. Chinese Traditional and Herbal Drugs, 2017, 48(7): 1298-1301.

[21] 孙辉, 杨金奎, 张克勤. 毛壳菌几丁质酶的酶学性质及抑菌活性研究[J]. 西北农林科技大学学报(自然科学版), 2015, (5): 168-173.

    SUN Hui, YANG Jinkun, ZHANG Keqin. Enzymatic properties and antibacterial activity of chitinase from Chaetomium sp. YMF 1.00843[J]. Journal of Northwest A﹠F Univereity, 2015, (5): 168-173.

[22] 卯婷婷, 陶刚, 赵玳琳, 等. 一株粪生毛壳菌的分离鉴定及其对辣椒枯萎病的防治效果[J]. 中国生物防治学报, 2017, 33(4): 552-560.

    MAO Tingting, TAO Gang, ZHAO Dailin, et al. Isolation and identification of coprophilous chaetomium strain and its biocontrol effect against pepper fusarium wilt[J]. Chinese Journal of Biological Control, 2017, 33(4): 552-560.

[23] 张素平, 田叶韩, 王建明, 等. 毛壳菌肥对黄瓜生长、产量和品质及氮磷钾吸收的影响[J]. 中国农学通报, 2017, (11): 35-42.

    ZHANG Suping, TIAN Yehan, WANG Jianming, et al. Effect of chaetomium fungal fertilizer on growth, production, quality of cucumber and its absorption of nitrogen, phosphorus and kalium[J]. Chinese Agricultural Sciences Bulletin, 2017, (11): 35-42.

[24] YU Haiying, PENG Wenying, MA Xiu, et al. Effects of no tillage on soil water content and physical properties of spring corn field in semiarid region of northern China[J]. Chinese Journal of Applied Ecology, 2011, 22(1): 99-104.

[25] 高云超, 朱文珊, 陈文新.秸秆覆盖免耕土壤真菌群落结构与生态特征研究[J]. 生态学报, 2001, 21(10): 1704-1710.

    GAO Yunchao, ZHU Wenshan, CHEN Wenxin. Community structures of saprophytic soil microfungi in three differently cultivated field soils in the North of China[J].Acta Ecologica Sinica, 2001, 21(10): 1704-1710.

[26] WANG Yin, XU Jie, SHEN Jianhua. Tillage, residue burningand crop rotation alter soil fungal community and water stable aggregation in arable fields[J]. Soil & Tillage Research, 2010, 107: 71-79.

[27] 张东升, 蒋先军, 袁俊吉. 垄作免耕对土壤团聚体中微生物生物量N和脲酶活性的影响[J].中国农学通报, 201l, 27(8): 254-258.

    ZHANG Dongsheng, JIANG Xianjun, YUAN Junji. Ridge tillage on soil aggregates microbial biomass N and urease activity[J]. Chinese Agricultural Science Bulletin, 2011, 27(8): 254-258.

陈力力, 刘金, 李梦丹, 张立钊, 邹应斌, 黄敏. 水稻-油菜双序列复种免耕、翻耕土壤真菌多样性[J]. 激光生物学报, 2018, 27(1): 60. CHEN Lili, LIU Jin, LI Mengdan, ZHANG Lizhao, ZOU Yingbin, HUANG Min. Diversity of Filamentous Fungus Community in Paddy Fields with Different Tillage Methods[J]. Acta Laser Biology Sinica, 2018, 27(1): 60.

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