UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析

习岗; 刘锴; 张晓辉; 李少华

光子学报, 2010, 39 (8): 1449, 网络出版: 2010-09-25

UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析

Dynamic Analysis of Ultra-weak Photon Radiation of Soybean Callus Induced by UV-B Radiation

论文大纲

习岗 ¹刘锴 ¹张晓辉 ²李少华 ¹

作者单位

¹ 西安理工大学理学院应用物理系，西安 710048

² 西安理工大学自动化与信息工程学院电子系，西安 710048

超弱光子辐射大豆愈伤组织 UV-B辐射细胞状态参量 Ultra-weak photon emission Soybean callus UV-B radiation State parameter of cell

摘要

为了研究细胞超弱光子辐射的动力学特征及其所揭示的生物学意义，用20 μW/cm2UV-B辐射大豆愈伤组织2 h，测定停止辐射后4 d内大豆愈伤组织在LED光诱导下的延迟发光.通过建立延迟发光动力学方程和数学拟合得到了大豆愈伤组织超弱光子辐射中的延迟发光积分强度、初始光子数、衰减参数和自发发光，讨论了这些发光动力学参数的生物学意义.研究结果表明，在停止UV-B辐射后的4 d内，大豆愈伤组织的光诱导延迟发光服从双曲线弛豫.动力学分析发现，延迟发光积分强度和初始光子数随处理后时间的进行呈现波动变化，停止UV-B辐射后，自发发光和丙二醛(MDA)含量均呈现升高的趋势，在辐射后2 d附近达到峰值，此后同步下降.用延迟发光积分强度和自发发光的比值定义细胞的状态参量Q和序参量R，发现UV-B辐射后大豆愈伤组织细胞Q值或R值的变化反映了UV-B辐射对大豆愈伤组织细胞的损伤以及细胞的恢复过程.

Abstract

In order to study the dynamic characteristics of ultra-weak photon emission in cell and its biological significance,soybean callus are radiated by UV-B radiation with 20μW/cm2 for 2 hours.Delayed luminescence of soybean callus induced by light within 4 days after the UV-B radiation is determined.Delayed luminescence integral intensity,the initial photon number,attenuation parameters and the spontaneous luminescence in ultra-weak photon emission of soybean callus are obtained through the establishment of delayed luminescence dynamics equation and mathematical fitting.The biological significance of these dynamic parameters of ultra-weak photon emission is discussed.The results show that delayed luminescence induced by light of soybean callus within 4 days after UV-B radiation to obey the laws of hyperbolic relaxation.Dynamic analysis shows that the changes of the delayed luminescence integral intensity and initial photon number with the time after UV-B treatment are volatility.The intensity of spontaneous luminescence and the content of malondialdehyde(MDA) are increased after the cessation of UV-B radiation.The peaks of them appear nearly two days after UV-B radiation.Using the ratio of delayed luminescence integral intensity and spontaneous luminescence to define the state parameter Q and the order parameter R of cell,it is found that the changes of Q value or R value of soybean callus cells after UV-B radiation reflect the damage of UV-B radiation on soybean callus and cell recovery process.

参考文献

[1] . Biophoton emission:New evidence for coherence and DNA as source[J]. Cell Biophys, 1984, 6(1): 33-52.

[2] . Biophoton distress flares signal the onset of the hypersensitive reaction[J]. Trends Plant Sci, 2005, 10(7): 307-309.

[3] . Changes in ultraweak photon emission and heart rate variability of epinephrine-injected rats[J]. Gen Physiol Biophys, 2005, 24(2): 147-159.

[4] . Ultraweak photon emission from herbivory-injured maize plants[J]. Naturwissenschaften, 2006, 93(1): 38-41.

[5] SLAWINSKI J,EZZAHIR A,GODLEWESKI M,et al.Stress-induced photon emission from perturbed organism ［J］.Experientia,1992,48(11-12):1041-1058.

[6] . Further analysis of delayed luminescence of plants[J]. Photochemistry and Photobiology B:Biology, 2005, 78(3): 235-244.

[7] . Spontaneous ultraweak photon emission from rice(Oryza sativa L.)and paddy weeds treated with a sulfonylurea herbicide[J]. Pesticide Biochemistry and Physiology, 2007, 89(2): 158-162.

[8] 顾樵.生物光子学［M］.北京:科学出版社,2007:9-12.

[9] 张玲瑞,邢达,王俊生,等.植物光诱导延迟荧光的紫外辐射环境胁迫检测［J］.光电子·激光,2007,18(7):878-881.

ZHANG Ling-rui,XING Da,WANG Jun-sheng,et al.Light-induced delayed fluorescence as an indicator for UV-B radiation environment stress on plants［J］.Optoelectronics Laser,2007,18(7):878-881.

[10] 习岗，卢洪，杨意,等.UV-B诱导的大豆愈伤组织异黄酮和超弱发光的变化,光子学报，2009，38(10)：2640-2644.

XI Gang，LU Hong,YAN Yi,et al.Changes of isoflavones and ultraweak photoemission of soybean callus induced by UV-B radiation［J］.Acta Photonica Sinica,2009，38(10)：2640-2644.

[11] . Spontaneous photon emission and delayed luminescence of two types of human lung cancer tissues:adenocarcinoma and squamous cell carcinoma[J]. Cancer Letters, 2005, 229(2): 283-289.

[12] . Enhancement of ultraweak photon emission with 3 MHz ultrasonic irradiation on transplanted tumor tissues of mice[J]. Ultrasonics Sonochemistry, 2008, 15(5): 921-926.

[13] 张志良,瞿伟菁.植物生理学实验指导［M］.北京:高等教育出版社,2003:274-243.

[14] . 紫外线B对水稻叶组织中活性氧代谢及膜系统的影响[J]. 植物生理学报, 1996, 22(4): 373-378.

. Effects of ultraviolet-B radiation on active oxygen metabolism and membrane system of rice leaves[J]. Acta Phytophysiologica Sinica, 1996, 22(4): 373-378.

[15] . 生存胁迫-紫外辐射增强对植物的生理生态效应[J]. 自然杂志, 2001, 23(4): 199-203.

. The survival stress ecophysiological effect of enhanced ultraviolet-B radiation on plant[J]. Chinese Journal of Nature, 2001, 23(4): 199-203.

[16] 张玲瑞,邢达,王俊生,等.利用光诱导延迟荧光的植物盐胁迫生理监测［J］.光子学报,2007,36(12):2284-2289.

ZHANG Ling-rui,XING Da,WANG Jun-sheng,et al.Light-induced delayed fluorescence as an indicator for the effects of salt stress on plant physiology［J］,Acta Photonica Sinica,2007,36(12):2284-2289.

[17] 李瑛,许文海,王俊生,等.基于非饱和光诱导延迟荧光的光合能力检测［J］.光电子·激光,2009,20(7):916-919.

LI Ying,XU Wen-hai,WANG Jun-sheng,et al.Detection of plant photosynthetic capacity based on unsaturated light-induced delayed Fluorescence［J］.Journal of Optoelectronics·Laser,2009,20(7 ):916-919.

习岗, 刘锴, 张晓辉, 李少华. UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析[J]. 光子学报, 2010, 39(8): 1449. XI Gang, LIU Kai, ZHANG Xiao-hui, LI Shao-hua. Dynamic Analysis of Ultra-weak Photon Radiation of Soybean Callus Induced by UV-B Radiation[J]. ACTA PHOTONICA SINICA, 2010, 39(8): 1449.

UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析

关于本站 Cookie 的使用提示

全站搜索

UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索