光电工程, 2015, 42 (1): 6, 网络出版: 2015-01-26
表面贴附微透镜阵列的OLED诱导荧光检测系统
OLED-induced Fluorescence Detection System Affixed with Micro Lens Array for Capillary Electrophoresis Microchip
微透镜阵列 有机发光二极管光源 毛细管电泳芯片 荧光检测 micro lens array organic light emitting diode capillary electrophoresis microchip fluorescence detection
摘要
为进一步减小光源的体积、简化光路系统结构,消除毛细管电泳芯片诱导荧光检测系统中激发光源的反射光和杂散光的干扰,设计并建立了垂直层叠结构的有机发光二极管诱导荧光检测系统。针对有机发光二极管器件发光强度较低的问题,采用在器件的玻璃基底表面贴附微透镜阵列薄膜的方法,提高了入射光的强度。在对CCD积分时间、PDMS 微透镜直径及两偏振片间的偏振角度等参数进行优化之后,利用检测系统实现了罗丹明B 样品溶液的毛细管电泳分离。
Abstract
A cross-polarization scheme and vertical multi-layer structure is presented to filter out the excitation light from the emission spectrum of fluorescent dyes using green organic light emitting diode as light source and a linear charge coupled device as intensity detector. Micro lens array film was affixed on glass base of organic light emitting diode in order to improve the incident intensity. Electrophoretic separation were performed with Rhodamine B as sample solution under the optimal parameters including integral time of CCD, diameter of PDMS micro lens and two polarizers’ polarization angle in the detection system.
杨晓博, 闫卫平. 表面贴附微透镜阵列的OLED诱导荧光检测系统[J]. 光电工程, 2015, 42(1): 6. YANG Xiaobo, YAN Weiping. OLED-induced Fluorescence Detection System Affixed with Micro Lens Array for Capillary Electrophoresis Microchip[J]. Opto-Electronic Engineering, 2015, 42(1): 6.