激光散斑衬比成像(LSCI)技术广泛应用于大视场的组织表层血流成像,当需要实时在体监测生物体深层组织或腔内组织的血流分布及变化时,将LSCI与内镜成像技术结合是解决LSCI成像深度问题的有效途径。为此,搭建了商用腹腔镜LSCI成像系统,并对微流体仿体和兔子大肠进行成像。实验结果表明,所搭建的系统可以校正静态散射以及消除系统噪声对散斑衬比度的影响,利用单次曝光下的散斑衬比测量值可以实现血流的定量监测,该商用腹腔镜LSCI成像系统将具有重要的临床应用潜力。

Laser speckle contrast imaging (LSCI) technology is widely used in tissue surface blood flow imaging with large field of view. When real-time in vivo monitoring of blood flow distribution and changes in deep tissue or lumen tissue is needed, the combination of LSCI and endoscopic imaging is an effective way to solve the problem of LSCI imaging depth. For this reason, a commercial laparoscopic LSCI imaging system is built to image microfluidic replicas and rabbit large intestine. The experimental results show that the system can correct the static scattering and eliminate the influence of system noise on speckle contrast, and the quantitative monitoring of blood flow can be realized by using the speckle contrast measurement under single exposure. The commercial laparoscopic LSCI imaging system will have important clinical application potential.