低水平激光联合Dycal用于大鼠直接盖髓后牙髓中SP、ALP的表达

赵举红; 张超杰; 齐元莉; 祖木热提古丽·阿不来提; 徐江

doi:doi:10.14128/j.cnki.al.20214104.930

应用激光, 2021, 41 (4): 930, 网络出版: 2022-01-10

低水平激光联合Dycal用于大鼠直接盖髓后牙髓中SP、ALP的表达

An Experimental Study on the Expression of SP and ALP in Rat Pulp After Direct Pulp Capping by Low Level Laser+Dycal

论文大纲

赵举红 ^*张超杰齐元莉祖木热提古丽·阿不来提徐江

作者单位

石河子大学第一附属医院口腔科，新疆石河子 832000

低水平激光直接盖髓术神经肽P物质（SP）碱性磷酸酶（ALP） low level laser Dycal Dycal direct pulp capping substance P (SP) alkaline phosphatase (ALP)

摘要

为观察低水平激光(LLL)联合Dycal用于大鼠磨牙直接盖髓，探讨不同时空牙髓内神经肽P物质（SP）和碱性磷酸酶（ALP）的表达，将 64只成年雄性大鼠随机分成正常对照组（ZC）、低水平激光组（LLL）、Dycal组（Dy）、联合组（LLL+Dy）。每组16只，以左侧上下颌第一磨牙为试验牙进行机械穿髓后行直接盖髓术，术后第3、7、14、28天取标本，行SP、ALP免疫组织化学染色，并测量其平均光密度值。结果显示：通过双因素单变量方差统计分析，术后第7、14天，SP、ALP表达存在显著性差异。术后第14天，SP表达达到峰值，且LLL+Dy组>Dy组>LLL组>ZC组（P<0.05）。术后第7天，LLL+Dy组和LLL组ALP表达水平达峰值，且LLL+Dy组>LLL组>Dy组>ZC组（P<0.05）。术后第28天，SP、ALP表达随着修复性牙本质形成逐渐降低至正常水平。结论：低水平激光联合Dycal对牙髓损伤后的修复及修复性牙本质的形成，可能与增强牙髓修复程序中SP和ALP的表达有关。

Abstract

In order to observe the exprssion of SP and ALP in rat pulp after direct pulp capping by low level laser +Dycal, 64 SD rats were randomly divided into 4 groups (n=16):Normal control group (ZC group), low level laser group (LLL group), Dycal group (Dy group) and low level laser+Dycal group (LLL+Dy group). The first molars of the left maxillary and mandibular of the rats mechanical wear pulp after direct pulp capping. SP and ALP expression in pulp tisssue was measured with immunohistochemical staining in different time. The mean optical density (MOD) of each specimen was measured. The results show that: According to the statistical analysis of two-factor univariate variance, there was a significant difference in SP expression on the 7th and 14th day after surgery. SP expression reached its peak and LLL+Dy group>Dy gruop>LLL group>ZC group(P<0.05) on the 14th day. ALP expression reached its peak and LLL+Dy group>LLL group>Dy group>ZC group (P<0.05) on the 7th day. SP and ALP expression gradually decreased to normal level with the formation of repaired dentin on the 28th day. Conclusion: The low level laser and Dycal can promote the repair of pulp injury and the formation of restorative dentin, which may be related toinduction of the expression of SP and ALP.

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赵举红, 张超杰, 齐元莉, 祖木热提古丽·阿不来提, 徐江. 低水平激光联合Dycal用于大鼠直接盖髓后牙髓中SP、ALP的表达[J]. 应用激光, 2021, 41(4): 930. Zhao Juhong, Zhang Chaojie, Qi Yuanli, Zumuretiguli Abulaiti, Xu Jiang. An Experimental Study on the Expression of SP and ALP in Rat Pulp After Direct Pulp Capping by Low Level Laser+Dycal[J]. APPLIED LASER, 2021, 41(4): 930.

低水平激光联合Dycal用于大鼠直接盖髓后牙髓中SP、ALP的表达

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