脉冲CO2激光照射对牙髓细胞影响的实验研究
目的动态观察脉冲CO2激光照射大鼠磨牙后牙髓组织中多种细胞因子在不同时间段和部位的表达,探讨热损伤后牙髓的防御和自我修复机制.方法采用免疫组织化学染色方法观测能量密度为10.5 J/mm2的脉冲CO2激光照射牙本质过敏症模型大鼠磨牙冠部牙髓组织中热休克蛋白70(HSP-70)、转化生长因子β1(TGF-β1)和骨形成蛋白2(BMP-2)在照射后第4小时,3d,2和4周时的表达,并对其进行定量分析.结果 HSP-70、TGF-β1,、BMP-2分别在照射后第4小时、2和4周表达最强,与其余各时间段的表达差异有显著意义.结论HSP-70、TGF-β1和BMP-2在损伤早期起防御作用,后期参与牙髓的自我修复过程,并促进修复性牙本质的形成.Laser Irradiation on Dental Pulp
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