研究了经三种不同类型的透明剂处理后的在体人皮组织在波长为400～1 000 nm范围内随时间变化反射光谱特性的变化及各组于580 nm处分别在0～60 min时间段各组反射光谱的改变程度。 实验采用USB-4000光纤光谱仪分别获取经丙三醇、 葡萄糖及丙二醇处理在体人皮肤组织前后的在0, 10, 20, 30, 40, 50和60 min的平均漫反射光谱。 实验结果表明: 分别经三种不同类型透明处理后的皮肤组织的平均反射光强度都呈现出明显的减弱， 但是随着各自作用于皮肤组织时间的增长， 所导致的相对反射率减少量逐渐增大。 经40%甘油作用于皮肤组织后在10, 20, 30, 40， 50和60 min处的相对反射率的减少分别为5%, 7%, 9%, 10%, 11%和12%， 经40%葡萄糖作用于皮肤组织后在10, 20, 30, 40, 50和 60 min处的相对反射率的减少分别为9%, 13%, 16%, 19%, 21%和22%， 而40%丙二醇作用于皮肤组织后在10, 20, 30, 40, 50和60 min处的相对反射率的减少分别为14%, 22%, 29%, 32%, 34%和35%。 可见， 这三种不同类型的透明剂都能够显著增强光在皮肤组织中的穿透能力， 在0～60 min的时间内各组得光透明时间进程也有所不同， 在同体积分数下， 光学透明效果差异显著地由高到低依次为40%丙二醇， 40%葡萄糖， 40%甘油。

The changes of skin tissue reflectance spectroscopy before and after being treated with the optical clearing agents of three different types of optical clearing within the wavelength rang of 400～1 000 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0～60 min at 580 nm in vivo were real-time dynamically researched. The reflectance spectroscopy of skin tissue before and after being dealt by the optical clearing agents of glycerol, glucose and propylene glycol was measured using a USB-4000 fiber spectrophotometer at 0, 10, 20, 30, 40, 50 and 60 min. The results showed that the reflectance spectral intensity was distinctly decreased, but the reflectance was significantly increased gradually with the time prolonged. However, different optical clearing agents have different clearing progress. The relative decrease of reflectance of palm skin tissue before and after being dealt by the optical agents of 40% glycerol, 40% glucose and 40% propylene glycol during 10, 20, 30, 40, 50 and 60 min at the wavelength 580 nm is 5%, 7%, 9%, 10%, 11% and 12%, 9%, 13%, 16%, 19%, 21% and 22%, and 14%, 22%, 29%, 32%, 34% and 35%, respectively. The significant improvement in light transmittance and enhancement of light penetration through tissue was demonstrated for all solutions. The effect and processes of optical clearing of skin tissue is not only closely related to the choosing of the clearing agent type, but also related to the treatment time with the skin tissue. The clearing progress of different type optical clearing agent showed the order of 40% propylene glycol, 40% glucose and 40% glycerol.