用光谱法研究光敏剂血卟啉单甲醚对肿瘤细胞光动力作用的分子机制

测定了光敏剂血卟啉单甲醚对人宫颈癌细胞HeLa光敏作用后的傅里叶红外光谱。结果显示: 光敏作用后, HeLa细胞磷酸二酯基团的对称伸缩振动峰1 085 cm-1和不对称伸缩振动峰1 246 cm-1蓝移, 强度下降; 蛋白质酰胺I带1 656 cm-1发生蓝移, 酰胺Ⅱ带1546 cm-1出现红移; CH2对称伸缩振动峰2 858 cm-1, 峰位蓝移2 cm-1, 峰值明显减弱; 细胞的蛋白质和核酸谱峰面积比值D1085/D1546降低。提示细胞中的DNA、蛋白质和磷脂结构受到损伤。结果表明: DNA、蛋白质和磷脂是血卟啉单甲醚光敏作用的主要靶分子。

Abstract

The paper determines the Fourier transform infrared spectrum of HeLa human cervix cancer cells photosensitized by the photosensitizer (Hematoporphyrin monomethylether). Results are showed: after photosensitizing, the peaks of symmetric stretching vibrations(1 085 cm-1) and asymmetric stretching vibrations(1246cm-1) of the phosphodiester bond of HeLa cells both shift to high wave number, with decreasing in peak strength; the protein amide I band(1 656 cm-1) shifts to blue wave and the protein amide II(1 546cm-1) band shifts to red wave; the peak (2 858 cm-1 ) of CH2 symmetric stretching vibrations(2 858 cm-1 )shifts 2cm-1 to blue wave, meanwhile, the peak strength decreases significantly; the integral ratio (D1086/D1540) of the peak area of protein and nucleic acids of HeLa cells goes down as well. Consequently, the conformations of DNA, protein, and phosphoric fat in HeLa cells are damaged to some extents. It is concluded that DNA, protein, and phosphoric fat are the main target molecules of photodymic effect of the photosensitizer (Hematoporphyrin monomethylether).

参考文献

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刘健, 郝倩云, 赵雪松, 刘婉华. 用光谱法研究光敏剂血卟啉单甲醚对肿瘤细胞光动力作用的分子机制[J]. 应用激光, 2012, 32(2): 159. Liu Jian, Hao Qianyun, Zhao Xuesong, Liu Wanhua. The Study on the Molecular Mechanisms of Photodynamic Effect of the Photosensitizer (HMME) Acting on Tumor Cell by Spectral Analysis[J]. APPLIED LASER, 2012, 32(2): 159.

用光谱法研究光敏剂血卟啉单甲醚对肿瘤细胞光动力作用的分子机制

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