1 厦门大学公共卫生学院分子影像暨转化医学研究中心，福建 厦门 361102
2 广东省医学科学院/广东省人民医院，医学研究部，光学分子影像实验室，广东 广州 510080
以针刺和拔罐疗法为代表的中医理疗被用于治疗肌肉骨骼疾病、缓解疼痛症状已有数千年的历史。中医理疗以疗效显著、安全性高的优势在全球流行。然而，传统的中医理论对于针刺疗法治疗机制的解释缺乏有效的科学证据，而且大多数针刺疗法的疗效缺乏有效的定量评估，无法保证其治疗效果及安全性。凭借高分辨率、高对比度、高安全性等特点，光学成像技术在针刺疗法的机制研究与疗效评估方面展现出了巨大潜力。目前用于研究针刺疗法的光学成像技术主要有激光多普勒血流灌注成像、激光散斑成像、近红外光谱成像和光声成像等，它们分别在针刺的血流动力学效应、大脑响应、治疗机制与疗效评估等方面取得了一定进展，但目前鲜见系统总结上述光学成像技术应用于针刺疗法研究的报道。鉴于此，本文系统介绍了这几类光学成像技术的特点以及它们在针刺疗法研究中的应用情况，并展望了其临床研究前景。
医用光学 针刺疗法 光学成像技术 激光多普勒 激光散斑成像 近红外光谱 光声成像
Author Affiliations
Abstract
1 State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China
2 State Key Laboratory of Precision Electronic Manufacturing Technology and Equipment, School of Electromechanical Engineering, Guangdong University of Technology, Guangzhou 510006, China
3 Jiangxi Key Laboratory of Optic-Electronic and Communication, Jiangxi Science and Technology Normal University, Nanchang 330038, China
Photoacoustic imaging has been developed to image the immune study at the macro scale. Macrophages play diverse roles in the acute response to infection and tissue repair. However, macrophages activities in acute inflammation at the microscopic level still remain challenging. In this work, we proposed optical-resolution photoacoustic microscopy to promptly monitor the labeled macrophages activities in normal and inflammatory groups. The result showed that many labeled macrophages emerged around the vessels firstly, then exuded into tissues, and finally disappeared in the inflammatory group injected with labeled macrophages. In summary, our method allows us to exactly image and track the immune cells of inflammatory diseases.
photoacoustic microscopy macrophages activities vessel parameter Chinese Optics Letters
2020, 18(12): 121701
