鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测

彭义杰; 刘木华; 赵进辉; 袁海超; 洪茜; 李耀

doi:doi:10.3788/lop51.103002

激光与光电子学进展, 2014, 51 (10): 103002, 网络出版: 2014-09-10

鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测下载： 504次

Detection of Chlortetracycline Residues in Egg Albumen Using Silver Colloids Surface-Enhanced Fluorescence of Eu (III)

论文大纲

彭义杰 ^*刘木华赵进辉袁海超洪茜李耀

作者单位

江西农业大学工学院/生物光电及应用重点实验室, 江西南昌 330045

光谱学金霉素荧光银胶 spectroscopy chlortetracycline fluorescence silver colloids Eu(III) Eu(III)

摘要

鸡蛋清中残留的金霉素(CTC)易进入人体内蓄积，从而对人体的健康产生损害。研究表明，银胶能作为表面基底来修饰金霉素与Eu(III)的配合物，进而使配合物的荧光强度得到显著性的增强。本研究应用银胶表面增强Eu(III)的荧光来检测鸡蛋清中金霉素的残留量，分别分析了不同胶体、银胶的加入量、Eu(III)的加入量、反应时间等因素对荧光强度的影响，并确定了最佳的实验条件，在最佳条件下建立了以617 nm 处的荧光特征峰强度(Y)与鸡蛋清中金霉素含量(X)之间的标定曲线。结果表明：鸡蛋清中金霉素浓度范围在1.5~29.5 mg/L 时，鸡蛋清中金霉素含量与荧光强度之间呈现良好的线性关系，线性方程为Y=5.2265X+24.033，决定系数R2 为0.9043，实测值与预测值之间的R2=0.9182。可见用银胶表面增强Eu(III)的荧光来快速检测鸡蛋清中金霉素的残留量是可行的。

Abstract

Chlortetracycline residues in egg albumen areaccumulated easily in humanbody, resulting in producing harm to human body. According to the research, silver colloids can be used as surface substrate to modify complexes of chlortetracycline and Eu(III), which makes the fluorescence intensity of complexes enhanced significantly. Silver colloids surface- enhanced fluorescence of Eu(III) is applied to detect chlortetracycline residues in egg albumen. Different addition amount of colloid and Eu(III), reaction time and other factors which have influence on fluorescence intensity are analyzed respectively in the research. The best experimental conditions are determined, and the calibration curve under the optimum condition is established between the fluorescence intensity of characteristic peak at 617 nm (Y) and chlortetracycline content (X). The results show that a good linearity is obtained between the fluorescence intensity and chlortetracycline concentration in egg albumen in the range of 1.5~29.5 mg/L. The linear function is Y=5.2265 + 24.033 X, and the determination coefficient is 0.9043. The determination coefficient between the measured values and predicted values is 0.9182. The results show that it is feasible to use silver colloids surface- enhanced fluorescence of Eu(III) to detect chlortetracycline residues in egg albumen rapidly.

参考文献

[1] 朴凤玉, 张小勇, 吴信子. 饲用金霉素在肉鸡饲养上的应用及检测[J]. 农业科技通讯, 2003. 24-25.

Pu Fengyu, Zhang Xiaoyong, Wu Xinzi. Feed aureomycin in the application and test of broiler feeding[J]. Animal Raising Technology, 2003. 24-25.

[2] 胡静.“速成鸡”离我们有多远 [J]. 科学之友(上旬), 2013. 76-77.

Hu Jing.“Crash chicken”how far away from us [J]. Friend of Science (Early), 2013. 76-77.

[3] 方忠意, 刘素梅, 宋志超, 等. 高效液相色谱法测定鸡蛋中4 种四环素类抗生素残留量[C]. 首届中国兽药大会动物药品学暨中国畜牧兽医学会动物药品学分会2008 学术年会, 2008. 221-224.

Fang Zhongyi, Liu Sumei, Song Zhichao, et al.. Determination of 4 tetracyclines in eggs by high performance liquid chromatography[C]. The First China Veterinary Congress Animal Medicine and Animal ChineseInstitute of Animal Husbandry and Veterinary Drug Association of the 2008 Symposium, 2008. 221-224.

[4] 宋戈, 郑伟. 高效液相色谱法测定乳及乳制品中多种四环素类药物残留[J]. 中国乳品工业, 2012, 40(8): 40-42.

Song Ge, Zheng Wei. Determination of various tetracycline antibiotics residues in milk and dairy products by high performance liquid chromatography[J]. Chinesedairy Industry, 2012, 40(8): 40-42.

[5] 付有利, 章建军, 贾艳, 等. 化学发光法测定蜂蜜中金霉素的残留含量[J]. 河南科学, 2011, 29(10): 1176-1180.

Fu Youli, Zhang Jianjun, Jia Yan, et al.. Determination on chlortetracycline residues in honey by chemiluminescence analysis[J]. Henan Science, 2011, 29(10): 1176-1180.

[6] 翟云忠, 吴建敏, 徐俊, 等. 鸡蛋中抗生素残留微生物法快速检测的研究[J]. 现代食品科技, 2008, 24(8): 839-841.

Zhai Yunzhong, Wu Jianmin, Xu Jun, et al.. Rapid detection of antibiotic residues in eggs by microbiological method[J]. Modern Food Science and Technology, 2008, 24(8): 839-841.

[7] 付云洁, 刘志国, 武玉香. 胶体金免疫层析法快速检测食品中金霉素残留[J]. 食品科学, 2010, 31(2): 191-194.

Fu Yunjie, Liu Zhiguo, Wu Yuxiang. Development of a one-step immunochromatographic strip test for rapid detection of chlortetracycline residue in food[J]. Food Science, 2010, 31(2): 191-194.

[8] 唐海波, 齐维, 唐时幸. 荧光定量法快速检测鲜奶中四环素类总量残留[J]. 现代食品科技, 2012, 28(11): 1600-1602.

Tang Haibo, Qi Wei, Tang Shixing. Rapid detection of tetracyclines residue in fresh milk using quantitative fluorescent assay[J]. Modern Food Science and Technology, 2012, 28(11): 1600-1602.

[9] 赵进辉, 袁海超, 刘木华, 等. 导数同步荧光法测定鸭蛋蛋清中金霉素残留含量[J]. 江西农业大学学报, 2013, 35(1): 0189-0194.

Zhao Jinhui, Yuan Haichao, Liu Muhua, et al.. Determination of aureomycin content in duck egg white with derivative synchronous fluorescence method[J]. Acta Agricultural Universitatis Jiangxiensis, 2013, 35(1): 0189-0194.

[10] 候法菊, 王玉宝, 苗延虹, 等. 荧光光度法研究修饰β -环糊精与Eu(III)-金霉素体系的相互作用及其应用[J]. 分析科学学报, 2011, 27(2): 231-234.

Hou Faju, Wang Yubao, Miao Yanhong, et al.. Fluorescence study of the interaction between the modified β-cyclodextrin and europium(III)- chlortetracycline system and its analytical application[J]. Journal of Analytical Science, 2011, 27(2): 231-234.

[11] 曹文丽, 于永丽, 徐淑坤, 等. 洛美沙星的铕离子荧光探针时间分辨荧光法测定[J]. 分析测试学报, 2010, 29(2): 147-151.

Cao Wenli, Yu Yongli, Xu Shukun, et al.. Determination of lcmefloxacin in using europium ion as fluorescent probe by time-resolved fluorometry[J]. Journal of Instrumental Analysis, 2010, 29(2): 147-151.

[12] M S Attia, Amr A Essawy, A O Yousset. Europium- sensitized and simultaneous pH- assisted spectrofluorimetric assessment of ciprofloxacin, norfloxacin and gatifloxacin in pharmaceutical and serum samples[J]. Journal of Photochemistry and Photobiology A: Chemistry, 2012, 236(15): 26-34.

[13] Hongliang tan, Yang Chen. Silver nanoparticle enhanced fluorescence of europium(III) for detection of tetracycline in milk[J]. Sensors and Actuators B, 2012, 173: 262-267.

[14] Lee P C, Meisel D. Adsorption and surface-enhanced Raman of dyes on silver and gold sols[J]. J Phys Chem, 1982, 86(17): 3991-3395.

[15] 徐良敏, 张正龙, 蔡晓燕, 等. 金属表面荧光增强的物理增强机制[J]. 发光学报, 2009, 30(3): 373-378.

Xu Liangmin, Zhang Zhenglong, Cai Xiaoyan, et al.. Physical mechanisms of fluorescence enhancement at metal surface [J]. Chinese Journal of Luminescence, 2009, 30(3): 373-378.

彭义杰, 刘木华, 赵进辉, 袁海超, 洪茜, 李耀. 鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测[J]. 激光与光电子学进展, 2014, 51(10): 103002. Peng Yijie, Liu Muhua, Zhao Jinhui, Yuan Haichao, Hong Qian, Li Yao. Detection of Chlortetracycline Residues in Egg Albumen Using Silver Colloids Surface-Enhanced Fluorescence of Eu (III)[J]. Laser & Optoelectronics Progress, 2014, 51(10): 103002.

鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测下载： 504次

关于本站 Cookie 的使用提示

全站搜索

鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测 下载： 504次

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索

鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测下载： 504次