Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution

Ali Jaafar; Malik H. Mahmood; Roman Holomb; Laszló Himics; Tamas Vaczi; Anton Y. Sdobnov; Valery V. Tuchin; Miklós Veres

doi:doi:10.1142/s1793545821420037

Journal of Innovative Optical Health Sciences, 2021, 14 (5): 2142003, Published Online: Dec. 6, 2021

Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution

论文大纲

Ali Jaafar ^1,2,3,*Malik H. Mahmood ^1,2,4Roman Holomb ^1,5Laszló Himics ¹Tamas Vaczi ¹Anton Y. Sdobnov ^6,7Valery V. Tuchin ^6,8,9Miklós Veres ¹

Author Affiliations

¹ Institute for Solid State Physics and Optics Wigner Research Centre for Physics P.O. Box 49, H-1121 Budapest, Hungary

² Institute of Physics, University of Szeged Dom ter 9, H-6720 Szeged, Hungary

³ Ministry of Higher Education and Scientific Research Baghdad 10065, Iraq

⁴ Physiology Department, College of Medicine University of Misan, Al-Amarah, Misan 62001, Iraq

⁵ Uzhhorod National University, Uzhhorod 88015 Transcarpathia, Ukraine

⁶ Science Medical Center, Saratov State University 83 Astrakhanskaya Str., Saratov 410012, Russia

⁷ Optoelectronics and Measurement Techniques Laboratory University of Oulu, 90570 Oulu, Finland

⁸ Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precision Mechanics and Control of the Russian Academy of Sciences 24 Rabochaya, Saratov 410028, Russia

⁹ Interdisciplinary Laboratory of Biophotonics National Research Tomsk State University 36 Lenin Avenue, Tomsk 634050, Russia

CRM skin collagen types I and IV amide III tissue optical clearing glycerol DMSO

Abstract

Confocal Raman microspectroscopy (CRM) with 633- and 785-nm excitation wavelengths combined with optical clearing (OC) technique was used for ex-vivo study of porcine skin in the Raman fingerprint region. The optical clearing has been performed on the skin samples by applying a mixture of glycerol and distilled water and a mixture of glycerol, distilled water and chemical penetration enhancer dimethyl sulfoxide (DMSO) during 30 min and 60 min of treatment. It was shown that the combined use of the optical clearing technique and CRM at 633nm allowed one to preserve the high probing depth, signal-to-noise ratio and spectral resolution simultaneously. Comparing the effect of different optical clearing agents on porcine skin showed that an optical clearing agent containing chemical penetration enhancer provides higher optical clearing efficiency. Also, an increase in treatment time allows to improve the optical clearing efficiency of both optical clearing agents. As a result of optical clearing, the detection of the amide-III spectral region indicating well-distinguishable structural differences between the type-I and type-IV collagens has been improved.

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Ali Jaafar, Malik H. Mahmood, Roman Holomb, Laszló Himics, Tamas Vaczi, Anton Y. Sdobnov, Valery V. Tuchin, Miklós Veres. Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution[J]. Journal of Innovative Optical Health Sciences, 2021, 14(5): 2142003.

Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution

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