g-C3N4@C-TiO2纳米颗粒增强可见光驱动的体外光动力灭活HL60细胞的试验研究

方杰; 王健; 张启云; 李淼淼; 肖睦沧; 艾保全; 熊建文

doi:doi:10.3969/j.issn.1007-7146.2021.01.006

激光生物学报, 2021, 30 (1): 43, 网络出版: 2021-08-23

g-C₃N₄@C-TiO₂纳米颗粒增强可见光驱动的体外光动力灭活HL60细胞的试验研究

An Experimental Study Based on g-C₃N₄@C-TiO₂ Nanoparticles to Enhance Visible-light-driven Photodynamic Therapy for HL60 Cells in vitro

论文大纲

方杰王健张启云李淼淼肖睦沧艾保全熊建文 ^*

作者单位

华南师范大学物理与电信工程学院, 广州 510006

光动力治疗光敏剂 HL60细胞肿瘤细胞凋亡 photodynamic therapy g-C3N4@C-TiO2 g-C3N4@C-TiO2 photosensitizer HL60 cells apoptosis of tumor cells

摘要

通过化学气相沉积法制备了g-C3N4@C-TiO2纳米颗粒, 利用X射线衍射(XRD)、荧光光谱(FS)、透射电子显微镜(TEM)、紫外-可见吸收光谱(UV-Vis)、能谱(EDS)等对纳米颗粒进行表征。分别研究了暗室条件及光照条件下g-C3N4@C-TiO2纳米颗粒对HL60细胞的作用效果。采用CCK-8法探究了一系列质量浓度梯度的纳米颗粒处理HL60细胞后的存活率, 并且通过荧光探针标记技术检测细胞内活性氧水平。试验结果表明, 在C-TiO2表面包裹g-C3N4, 可将TiO2可吸收的光波长范围拓展至可见光波段。制备的g-C3N4@C-TiO2粒径在10～20 nm, 满足其进入细胞的尺寸要求。暗毒性试验表明g-C3N4@C-TiO2纳米颗粒在暗室条件下对细胞的毒性较小, 证明了其具有良好的生物相容性。同时, 与TiO2和C-TiO2纳米颗粒处理组相比, g-C3N4@C-TiO2处理组的光动力灭活效率高达(76.5±1.9)%, 表明其可能成为治疗白血病的潜在光敏剂。

Abstract

C-doped TiO2 nanoparticles combined with g-C3N4 were prepared by chemical vapor deposition, using X-ray diffraction, fluorescence spectroscopy, transmission electron microscopy, UV-Vis absorption spectroscopy and energy dispersive spectrometer to characterize nanoparticles. The effects of g-C3N4@C-TiO2 nanoparticles on HL60 cells in vitro were explored in dark and under light irradiation. Cell Counting Kit-8 (CCK-8) method was used to investigate the viability of HL60 cells treated with nanoparticles with a series of concentration gradients, and the level of intracellular reactive oxygen species was detected via fluorescent probe labeling technology. The experimental results showed that by modifying g-C3N4 on the surface of C-TiO2, the absorption wavelength range of TiO2 could be extended to the visible band. The particle size of g-C3N4@C-TiO2 prepared was about 10～20 nm, which met the size requirement of entering cells. Dark toxicity experiment proved that g-C3N4@C-TiO2 nanoparticles were less toxic to cells under darkroom conditions, which suggested they had good biocompatibility. Meanwhile, compared with the groups treated?with?TiO2 and C-TiO2?nanoparticles, the photocatalytic inactivation efficiency of the group treated with g-C3N4@C-TiO2 was as high as (76.5±1.9)% under light irradiation, indicating its potential as a potential photosensitizer for the treatment of leukemia.

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方杰, 王健, 张启云, 李淼淼, 肖睦沧, 艾保全, 熊建文. g-C₃N₄@C-TiO₂纳米颗粒增强可见光驱动的体外光动力灭活HL60细胞的试验研究[J]. 激光生物学报, 2021, 30(1): 43. FANG Jie, WANG Jian, ZHANG Qiyun, LI Miaomiao, XIAO Mucang, AI Baoquan, XIONG Jianwen. An Experimental Study Based on g-C₃N₄@C-TiO₂ Nanoparticles to Enhance Visible-light-driven Photodynamic Therapy for HL60 Cells in vitro[J]. Acta Laser Biology Sinica, 2021, 30(1): 43.

g-C₃N₄@C-TiO₂纳米颗粒增强可见光驱动的体外光动力灭活HL60细胞的试验研究

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