Inactivation of bovine immunodeficiency virus by photodynamic therapy with HMME

Huijuan Yin; Yingxin Li; Zhaohui Zou; Wentao Qiao; Xue Yao; Yang Su; Hongyan Guo

Chinese Optics Letters, 2008, 6 (12): 944, Published Online: Dec. 17, 2008

Inactivation of bovine immunodeficiency virus by photodynamic therapy with HMME Download： 662次

论文大纲

Huijuan Yin ¹Yingxin Li ^1,*Zhaohui Zou ²Wentao Qiao ²Xue Yao ²Yang Su ²Hongyan Guo ¹

Author Affiliations

¹ Laser Medicine Laboratory, Biomedical Engineering Department of Tianjin Medical University, Tianjin 300070

² College of Life Sciences, Nankai University, Tianjin 300071

光动力疗法血卟啉单甲醚牛免疫缺陷病毒 170.5180 Photodynamic therapy 170.1530 Cell analysis 170.2520 Fluorescence microscopy

Abstract

To investigate the effect of photodynamic therapy (PDT) with hematoporphrin monomethyl ether (HMME) on bovine immunodeficiency virus (BIV) can provide the basis theory for photoinactivation of human immunodeficiency virus (HIV). To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-(4,5)-dimethylthiahiazol-2-y1-3,5-di-phenytetrazolium bromide (MTT) with power density of 5 and 25 mW/cm2 and energy density from 0.6 to 3 J/cm2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.

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Huijuan Yin, Yingxin Li, Zhaohui Zou, Wentao Qiao, Xue Yao, Yang Su, Hongyan Guo. Inactivation of bovine immunodeficiency virus by photodynamic therapy with HMME[J]. Chinese Optics Letters, 2008, 6(12): 944.

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