Author Affiliations
Abstract
1 Laser Medicine Laboratory, Biomedical Engineering Department of Tianjin Medical University, Tianjin 300070
2 College of Life Sciences, Nankai University, Tianjin 300071
To investigate the effect of photodynamic therapy (PDT) with hematoporphrin monomethyl ether (HMME) on bovine immunodeficiency virus (BIV) can provide the basis theory for photoinactivation of human immunodeficiency virus (HIV). To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-(4,5)-dimethylthiahiazol-2-y1-3,5-di-phenytetrazolium bromide (MTT) with power density of 5 and 25 mW/cm2 and energy density from 0.6 to 3 J/cm2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.
光动力疗法 血卟啉单甲醚 牛免疫缺陷病毒 170.5180 Photodynamic therapy 170.1530 Cell analysis 170.2520 Fluorescence microscopy Chinese Optics Letters
2008, 6(12): 944
