中国激光, 2023, 50 (9): 0907102, 网络出版: 2023-04-24
具有荧光开关特性的喹啉衍生物用于高效选择性标记细胞脂滴 下载: 562次
Quinoline Derivatives with Fluorescent Switching Properties for Efficient Selective Labeling of Cellular Lipid Droplets
图 & 表
图 1. 荧光探针的设计、合成与光物理性质。(a)Lipi-QL的分子结构及其TD-DFT计算结果;(b)Lipi-QL在不同极性有机溶剂中的吸收发射光谱;(c)Lipi-QL在不同二氧六环溶液中的荧光光谱及荧光照片;(d)BODIPY 493/503在不同二氧六环溶液中的荧光光谱及荧光照片
Fig. 1. Design, synthesis, and photophysical properties of fluorescent probes. (a) Molecular structure of Lipi-QL and its calculation results by TD-DFT; (b) absorption emission spectra of Lipi-QL in different polar organic solvents; (c) fluorescence spectra and fluorescence photographs of Lipi-QL in different dioxane solutions; (d) fluorescence spectra and fluorescence photographs of BODIPY 493/503 in different dioxane solutions
图 2. HeLa细胞中Lipi-QL和Ph-Red的共定位实验(标尺为10 μm)。(a)Lipi-QL的绿色成像通道;(b)Ph-Red的红色成像通道;(c)两个荧光通道以及明场的叠加图片
Fig. 2. Co-localization experiments of Lipi-QL and Ph-Red in HeLa cells (scale bar is 10 μm). (a) Green imaging channel of Lipi-QL; (b) red imaging channel of Ph-Red; (c) superimposed images of two fluorescence channels and bright field
图 3. 不同浓度下Lipi-QL与BODIPY 493/503在HeLa细胞中的染色选择性对比(标尺为10 μm)
Fig. 3. Comparison of staining selectivity between Lipi-QL and BODIPY 493/503 in HeLa cells under different concentrations (scale bar is 10 μm)
图 4. 使用Lipi-QL染色的HeLa细胞的三维共聚焦成像
Fig. 4. 3D confocal imaging of HeLa cells stained with Lipi-QL
图 5. 活Hela细胞的多色共聚焦成像(标尺为10 μm)。(a)Hoechst 33342染色的细胞核;(b)Lipi-QL染色的脂滴;(c)Lyso Tracker Red染色的溶酶体;(d)Mito Tracker Deep Red染色的线粒体;(e)四个荧光通道的合并图片
Fig. 5. Multicolor confocal imaging of live Hela cells ( scale bar is 10 μm). (a) Hoechst 33342-stained nucleus; (b) Lipi-QL-stained lipid droplets; (c) Lyso Tracker Red-stained lysosomes; (d) Mito Tracker Deep Red-stained mitochondria; (e) merged images of four fluorescence channels
赵力, 周日, 刘冠男, 彭桂衫, 王晨光, 贾晓腾, 卢革宇. 具有荧光开关特性的喹啉衍生物用于高效选择性标记细胞脂滴[J]. 中国激光, 2023, 50(9): 0907102. Li Zhao, Ri Zhou, Guannan Liu, Guishan Peng, Chenguang Wang, Xiaoteng Jia, Geyu Lu. Quinoline Derivatives with Fluorescent Switching Properties for Efficient Selective Labeling of Cellular Lipid Droplets[J]. Chinese Journal of Lasers, 2023, 50(9): 0907102.