Photoacoustic Doppler flow measurement based on continuous wave laser excitation owns the merit of clearly presenting the Doppler power spectra. Extending this technique to dual wavelengths can gain the spectral information of the flow sample extra to the flow speed information. An experimental system with two laser diodes respectively operated at 405 nm and 660 nm wavelengths is built and the flow measurement with black and red dyed polystyrene beads is performed. The measured Doppler power spectra can vividly reflect the flow speed, the flow direction, as well as the bead color. Since it is straightforward to further apply the same principle to multiple wavelengths, we can expect this type of spectroscopic photoacoustic Doppler flow measurement will be developed in the near future which will be very useful for studying the metabolism of the slowly moving red blood cell inside microvessels.

为了测量碳颗粒悬混溶液的流速矢量，将自相关方法引入了光声多普勒流速测量。纵向和横向速度分量分别由多普勒频移和多普勒频带展宽得到。光声信号由波长532 nm，重复频率20 Hz的脉冲激光激励，由中心频率10 MHz的点聚焦压电超声换能器采集。由微量注射泵驱动的碳颗粒悬混液模拟血液的流动。时域光声信号由希尔伯特变换为复信号后进行自相关计算。多普勒频移和多普勒频宽的标准偏差由若干独立A扫的自相关平均得到。对比之前采用序列A扫的互相关方法，自相关中的信号时移大小可自定义，避免了对高重复频率脉冲激光的要求，有利于探测深度的提高。该方法的可行性通过对流速为16-32 mm/s、多普勒角度为50°的碳颗粒悬混液流速矢量的测量得到了初步验证。

血液微循环的测量研究有着非常重要的临床诊断价值。现有的可用于血液微循环测量的光学多普勒技术可分为三大类: 激光多普勒技术、相干层析多普勒技术和光声多普勒技术。在介绍这三类技术的基本工作原理和主要特点的基础上, 简要分析了各类中最具代表性的前沿技术, 分别为激光多普勒宽场实时成像技术、光学相干层析多普勒微血管造影术和光声多普勒流速测量技术。对这些前沿技术的进一步发展和实际应用进行了展望。