报道了一种采用氧化石墨烯作为可饱和吸收体的二极管泵浦的被动调Q和调Q锁模运转的Tm, Ho: LLF激光器。采用透过率分别为3%、5%和9%的输出镜，首先研究了Tm, Ho: LLF激光器的连续运转特性。实验和模拟结果均表明采用透过率为9%的输出镜输出特性最好，当最大泵浦功率为20 W时，连续光输出功率高至1793 mW。接着以氧化石墨烯为饱和吸收体，采用透过率为9%的输出镜研究了Tm, Ho: LLF激光器的调Q和调Q锁模特性。实验表明：当790 nm LD泵浦功率小于7.26 W时，激光处于单纯调Q运转状态；当大于7.26 W时，激光器进入稳定的调Q锁模状态，当最大泵浦功率为20 W时，最大输出功率为1052 mW，锁模重复频率为53.19 MHz，对应的平均单脉冲能量为19.77 nJ，该单脉冲能量是目前2 μm锁模激光器的最高指标，同时证实了氧化石墨烯材料在大能量高功率激光锁模中是发展潜力优良的二维锁模材料。

采用垂直生长法制备的氧化石墨烯（Graphene oxide, GO）作为可饱和吸收体，利用典型“X”型折叠腔在全固态Tm:Lu₃Al₅O₁₂(Tm:LuAG)陶瓷激光器中实现了调Q锁模运转。以790 nm激光二极管(Laser diode, LD)作为泵浦源，当泵浦功率大于8 W时，激光器进入稳定的调Q锁模状态。当输出镜透过率为5%时，连续光最高输出功率为714 mW，斜效率为4.94%。当泵浦达到16 W时，激光器最大输出功率为200 mW , 光谱中心波长为2024 nm，脉冲宽度约为695 ps，对应的锁模脉冲重复频率为108.7 MHz，调Q包络中锁模脉冲的调制深度接近100%。该2 μm超短脉冲激光器在生物医学和激光通讯等领域具有非常重要的应用。

The aim of this study is to develop a novel technique for improving the intraoperative margin assessment of glioblastoma by examining the total extrinsic extracellular matrix (ECM) with eosin staining using fluorescence lifetime imaging microscopy (FLIM) and scale-invariant feature transform (SIFT) descriptor analysis. Pseudo-color FLIM images obviously exhibit ECM distributions, changes in sequential sections, and different regions of interest. Meanwhile, SIFT descriptors are first utilized for the discrimination of glioblastoma margins by matching similar ECM regions and extracting keypoint orientations from FLIM images obtained from a series of continuous slices. The findings indicate that FLIM imaging with SIFT analysis of the total ECM is a promising method for improving intraoperative diagnosis of frozen and surgically excised brain specimen sections.

Brain regenerative studies require precise visualization of the morphological structures. However, few imaging methods can effectively detect the adult zebrafish brain in real time with high resolution and good penetration depth. Long-term in vivo monitoring of brain injuries and brain regeneration on adult zebrafish is achieved in this study by using 1325 nm spectral-domain optical coherence tomography (SD-OCT). The SD-OCT is able to noninvasively visualize the skull injury and brain lesion of adult zebrafish. Valuable phenomenon such as the fractured skull, swollen brain tissues, and part of the brain regeneration process can be conducted based on the SD-OCT images at different time points during a period of 43 days.