Author Affiliations
Abstract
1 MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
2 Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
3 Department of Ophthalmology and Optometry, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China
We present for the first time, to the best of our knowledge, a needle probe for photoacoustic viscoelasticity (PAVE) measurements at a depth of 1 cm below the sample surface. The probe uses a gradient index rod lens, encased within a side-facing needle (0.7 mm outer diameter), to direct excitation light (532 nm) and detection light (1325 nm) focused on the sample, collecting and directing the returned detection light in a spectral domain low coherence interferometry system, which allows for obtaining optical phase differences due to photoacoustic oscillations. The feasibility of needle probe for PAVE depth characterization was investigated on gelatin phantoms and in vitro biological tissues. The experimental results in an in vivo animal model predict the great potential of this technique for in vivo tumor boundary detection.
needle probe photoacoustic viscoelasticity measurement depth-resolved mechanical phase delay mechanical interface Chinese Optics Letters
2022, 20(8): 081701
南京理工大学 电子工程与光电技术学院, 南京 210094
频谱编码显微镜是用衍射光栅和光谱分析装置来获得显微图像.样品上不同的位置被不同的波长照明, 通过对反射光光谱进行解码来得到空间信息.搭建了一个基于超连续光源和自制光谱仪的频谱编码显微成像系统, 其横向分辨率为1.72±0.13 μm(编码线方向)和1.26±0.08 μm(垂直于编码线方向), 测得不同横向位置处的轴向分辨率有差异.对离体猪肝组织不同部位进行了成像(可见血管、肝窦内皮细胞和肝细胞); 对鸡心组织以10 μm深度间隔进行成像, 测得不同深度处结构信息不一样.结果表明, 采用该频谱编码成像的方法能够进行高分辨的深度成像.
成像系统 显微镜 频谱编码 深度成像 分辨率 衍射 红外成像 Imaging system Microscopy Spectrally encoded Depth-resolved Resolution Diffractive optics Infrared imaging
Author Affiliations
Abstract
1 中国科学院上海光学精密机械研究所高功率激光物理联合实验室, 上海 201800
2 中国科学院大学, 北京 100049
In the single-beam illuminating 3PIE (ptychographic iterative engine) method, the specimen is vertically illuminated without considering the influence of oblique illumination on the reconstruction result. In this paper, a dual-beam illuminating 3PIE method is proposed on the basis of the single-beam illuminating 3PIE method, and the simulation result shows that the reconstructed images are influenced by the phase inclination factor of the initial guessed illumination light beam. In the process of iterative recovery by the proposed method, two light beams are used to update the complex amplitude distribution of the same specimen area. The proposed method is demonstrated theoretically and experimentally. The results verify that the method can improve the convergence speed while weakening crosstalk between different specimen slices, and it is suitable for three-dimensional depth resolved imaging.
成像系统 相干光学 相干衍射成像 层析 迭代算法 相位恢复 imaging systems coherence optics coherent diffraction imaging depth resolved imaging iterative algorithm phase retrieval Collection Of theses on high power laser and plasma physics
2016, 14(1): 0811002
1 中国科学院上海光学精密机械研究所高功率激光物理联合实验室, 上海 201800
2 中国科学院大学, 北京 100049
在单光束照明3PIE(Ptychographic Iterative Engine)方法中，照明光垂直照射在待测样品表面，没有考虑照明光倾斜对重构结果的影响。在单光束照明3PIE方法的基础上提出了双光束照明3PIE方法，同时模拟分析了初始猜测照明光中相位倾斜因子对成像质量的影响。双光束照明3PIE方法在迭代恢复样品的过程中，两束照明光均用于更新同一样品的复振幅分布，理论分析和实验结果均表明，双光束照明3PIE方法能够提高算法的收敛速度，有效减弱恢复样品不同层次之间的串扰，适用于三维层析成像。
成像系统 相干光学 相干衍射成像 层析 迭代算法 相位恢复
Author Affiliations
Abstract
1 Department of Biomedical Engineering, College of Engineering Peking University, Beijing 100871, P. R. China
2 Department of Applied Physics, College of Science Xi'an University of Technology, Xi'An 710048, P. R. China
3 College of Science, Shanghai Institute of Technology 100 Haiquan Road, Shanghai 201418, P. R. China
We report the virtual instrumentation of both time-domain (TD) and spectral-domain (SD) optical coherence tomography (OCT) systems. With a virtual partial coherence source from either a simulated or measured spectrum, the OCT signals of both A-scan and B-scan were demonstrated. The spectrometric detector's pixel number, dynamic range, noise, as well as spectral resolution can be simulated in the virtual spectral domain (SD-OCT). The virtual-OCT system provides an environment for parameter evaluation and algorithm optimization for experimental OCT instrumentation, and promotes the understanding of OCT imaging and signal post-processing processes.
Optical coherence tomography simulation LabVIEW depth-resolved imaging crosssectional imaging Journal of Innovative Optical Health Sciences
2014, 7(5): 1450030
1 天津大学精密测试技术及仪器国家重点实验室, 天津 300072
2 天津大学精密仪器与光电子工程学院生物医学工程系, 天津 300072
漫反射光谱人体成分检测研究中，光纤探头接收漫反射光子穿透深度及传输路径对检测效率及灵敏度影响较大，而传统的检测光纤探头无法实现对特定深度漫反射光子选择性接收。针对人体成分检测需要，提出了采用一定入射接收角度及半球透镜耦合的漫反射测量光纤探头结构。在三层皮肤模型基础上，结合光纤探头形式改进Monte Carlo程序，计算光纤探头接收漫反射光子穿透深度，有效光子比例，有效信息载荷以及真皮层检测灵敏度。结果表明，设计的光纤探头可实现真皮层漫反射光子选择性接收，检测效率以及测量光谱受非目标层组织结构及光学参数影响较小，可有效提高人体成分无创检测灵敏度。
医用光学 深度分辨光纤探头 蒙特卡洛 漫反射光谱 无创检测
1 中国科学院上海光学精密机械研究所, 上海 201800
2 中国科学院研究生院, 北京 100039
提出一种深度分辨的色散补偿方法，用于增强频域光学相干层析成像的纵向分辨率。将频域光学相干层析成像采集的干涉谱信号傅里叶变换到空域，获取对应不同深度的干涉谱条纹相位，通过对其进行调整实现对被测样品不同深度处的色散的精确补偿。避免传统方法中采用统一色散系数进行色散补偿所带来的过补偿与欠补偿误差，可以有效消除色散引起的频域光学相干层析成像系统点扩展函数的展宽和扭曲。模拟和实验结果表明，基于深度分辨的色散补偿方法在样品的全深度探测范围内可以达到较佳的补偿效果，可有效提高光学相干层析成像系统的纵向分辨率。
医用光学 生物技术 光学相干层析成像 色散补偿 深度分辨
