1 上海大学理学院 纳米科学与技术研究中心，上海 200444
2 上海大学理学院 化学系，上海 200444
3 上海大学 纳米化学与生物学研究所，上海 200444
设计并合成了一种用于近红外光驱动的化学?光热治疗的上转换?铋纳米体系诊疗剂（UBDAs），其具有出色的光热转换能力（28.5%）和良好的生物相容性。同时，在980 nm近红外光的激发下，UBDAs能够发射紫外/可见光，用于促进光敏剂偶氮苯在介孔中的连续旋转?翻转运动，从而实现药物的可控释放，且利用近红外光激发能够有效避免传统紫外光对生物组织的副作用。光热实验表明，UBDAs杂化纳米体系在980 nm激光照射下具有良好的光热效应。此外，含有Tm3+和Bi元素的UBDAs有望用于上转换发光成像和X射线计算机断层成像，进而实现双模成像介导且单一近红外光激发的癌症化学疗法和光热疗法。该研究结果为诊断和协同增强抗肿瘤治疗的综合研究提供了新的思路。
上转换发光 成像 光热治疗 药物释放 杂化纳米体系 upconversion luminescence imaging photothermal therapy drug release hybrid nanosystems
Author Affiliations
Abstract
1 Department of Orthopaedics Shanxi Medical University Second Hospital Taiyuan 030001, P. R. China
2 Institute of Materials Science and Technology Sichuan University, Chengdu 610065, P. R. China
Although the electrospinning technique has been devoted to promoting therapeutic purposes as a drug carrier, however, there are still many fundamental problems in this area. This work focuses on a comparison of various diameter polyethersulfone (PES) electrospun ultrafine fibers as antimicrobial materials. The fibrous morphology, antimicrobial agent distribution, thermally property, and biocompatibility evaluation of PES-based ultrafine fibers were systematically investigated. The results demonstrated that the PES-based ultrafine fibers were suitable as antimicrobial material. Furthermore, the drug release behavior and mechanism were studied through total immersion. The release mechanism was confirmed to Fickian diffusion. It was revealed that the drug max release amount (71.5%) and release rate (7.71) are the highest for the smallest diameter ultrafine fibers. Meanwhile, the antimicrobial activity of PES ultrafine fibers is also inversely correlated with the diameter of fiber. The electrospun PES fibers would control their release behavior through the diameter and have a potential application in the wound dressings, such as chronic osteomyelitis and exposure injury.
Electrospinning polyethersulfone ultrafine fibers drug release antimicrobial activity Journal of Innovative Optical Health Sciences
2021, 14(2): 2041005
湖南省人民医院老年医学研究所, 长沙 410016
利用金属-有机框架材料ZIF-8包裹二硫化钼(MoS2)纳米片和阿霉素(DOX)构建一种可通过酸性pH和近红外(NIR)光双触发的肿瘤化学/光热协同治疗体系。首先, 通过水热反应和超声处理制备粒径为~100 nm、厚度为0.3~1.4 nm的MoS2纳米片。然后, 通过一步法将可酸降解的金属-有机框架ZIF-8包裹在所制备的MoS2纳米片上, 并同时装载抗肿瘤药物DOX, 形成装载DOX的ZIF-8包裹MoS2纳米复合物(DOX/MoS2@ZIF-8)。将该纳米复合物应用到肿瘤细胞的化学/光热协同治疗: 当处于酸性条件(例如: 溶酶体中pH大约为5)和NIR激光(780 nm, 2.1 W/cm2)照射的情况下, DOX/MoS2@ZIF-8纳米复合物上包裹的ZIF-8金属-有机框架会发生酸降解, 释放出所包裹的DOX, 细胞质中的DOX可以进入细胞核中诱导细胞凋亡; 同时, MoS2纳米片能够将光能转换为热能, 光致高温同样能诱导细胞凋亡, 因此, 化学/光热协同肿瘤治疗得以实现。细胞存活率试验证明: 该DOX/MoS2@ZIF-8纳米复合物在SMMC-7721细胞上表现出良好的化学/光热协同治疗作用, 能够对肿瘤细胞进行高效地杀伤。
二硫化钼纳米片 金属-有机框架材料 类沸石咪唑酯骨架材料-8 化学/光热协同治疗 酸/近红外光双响应的药物释放 molybdenum disulfide nanosheets metal organic frameworks zeolitic imidazolate framework-8 chemo/photothermal synergistic therapy pH- and NIR laser dual-responsive drug release
华南师范大学激光生命科学研究所， 激光生命科学教育部重点实验室， 生物光子学研究院， 广东 广州 510631
肿瘤单一药物治疗的效果往往不佳，且易产生耐药性，因此，肿瘤的多药协同治疗具有明显优势，并逐渐引起重视。本工作基于具有良好生物相容性和生物可降解性的聚乙二醇-嵌段-聚乳酸(PEG-b-PLA)两嵌段聚合物，物理包埋两种化疗药物，阿霉素(Doxorubicin, DOX)和喜树碱(Camptothecin, CPT)，实现两种化疗药物的共传输、药物释放与协同给药，表现出良好的抗肿瘤活性。
聚乙二醇-嵌段-聚乳酸 生物可降解性 药物释放 协同给药 poly(ethylene glycol)-b-Poly(lactic acid) biodegradability drug release synergetic drug treatment
1 东北师范大学 物理学院， 吉林 长春130024
2 长春理工大学 理学院， 吉林 长春130022
以直径1 μm的脂质体为空化研究对象，从修正的Rayleigh空化方程入手，研究机械系数(MI)对300 kHz和1 MHz超声作用时空化效应的影响。脂质体的药物释放以超声作用前后脂质体中钙黄绿素的荧光强度为量度。模拟结果表明:在微泡振荡过程中，由超声波驱动产生的负向最大泡壁运动速度促使微泡半径从最大快速减小接近于零，微泡积聚到最大能量。对于300 kHz和1 MHz的激励超声，存在一个拐点(MI)值，当MI小于接近0.4时，1 MHz微泡半径变化幅度强于300 kHz; 当MI＞0.4时，300 kHz微泡半径变化幅度强于1 MHz。这一结果预示在此范围内，300 kHz的药物释放效果好于1 MHz。本研究为超声空化效应研究及超声药物释放应用提供了理论依据。
超声空化效应 脂质体药物释放 动力学行为 cavitation drug release of liposome microbubble dynamics
Author Affiliations
Abstract
1 Optical Sciences Group MESA+ Institute for Nanotechnology University of Twente, The Netherlands
2 Institute of Pharmaceutics and Biopharmaceutics Heinrich-Heine University of D¨usseldorf, Germany
3 Centre for Drug Research University of Helsinki, Finland
4 School of Pharmacy University of Otago, New Zealand
5 BioPhysical Engineering Group MESA+ Institute for Nanotechnology University of Twente, The Netherlands
Coherent anti-Stokes Raman scattering (CARS) microscopy is used to visualize the release of a model drug (theophylline) from a lipid (tripalmitin) based tablet during dissolution. The effects of transformation and dissolution of the drug are imaged in real time. This study reveals that the manufacturing process causes significant differences in the release process: tablets prepared from powder show formation of theophylline monohydrate on the surface which prevents a controlled drug release, whereas solid lipid extrudates did not show formation of monohydrate. This visualization technique can aid future tablet design.
Drug release coherent anti-Stokes Raman scattering (CARS) micro Journal of Innovative Optical Health Sciences
2009, 2(1): 37–43
