识别动脉粥样硬化斑块的易损性是防治急性冠状动脉疾病的重要途径。纤维帽厚度、脂质核心大小、管腔狭窄程度和管腔的力学特性是评估斑块易损性的关键参数。然而，单一模态的血管内成像技术难以通过一次成像获取用于评估斑块易损性的全面信息。本团队通过复用光路和声路，将血管内超声成像（IVUS）和血管内光学相干层析成像（IVOCT）与血管内光声成像（IVPA）、光声弹性成像（IVPAE）有机结合到一起，开发了一种血管内光声-超声-光学相干层析-光声弹性四模态一体化成像探头及成像系统。该一体化成像探头的成像直径仅为0.97 mm，光学相干层析、光声、超声模态的横向分辨率分别为20.5、61.3、122.2 μm，纵向分辨率分别为15.8、57.4、72.5 μm。离体模拟样品和兔腹主动脉的在体成像实验验证了血管内四模态成像能够提供血管壁的宏观和微观结构信息，同时能够特异性识别脂质成分和反映脂质斑块的弹性力学信息。该一体化探头可一次性获取血管内斑块的多物理影像特性，有望为动脉粥样硬化斑块的深入理解和诊治提供新型的介入成像方法和工具。

为了实现生物组织化学特性与物理结构的同时测量,提出了结合光学相干层析扫描(OCT)成像与荧光比率成像的双模态内窥探头。根据OCT成像原理,确定OCT系统的中心波长为1300 nm;根据所选用pH指示剂(SNARF-1)的吸收光谱与反射光谱,确定荧光的激发波长为520 nm。根据ABCD矩阵计算并确定探头的基本结构,基于理论计算结果进行光学元件的加工与探头的组装。此外,搭建了双模态系统,采用该系统测量了探头的横向分辨率与工作距离。通过猪大肠验证了探头同时进行成像和pH检测的能力,实验结果表明:探头能同时进行OCT成像与pH测量,并且具有较高的精度。OCT成像在空气中的横向分辨率约为37.3 μm,工作距离约为13.4 mm,在生物组织中可以实现精度为0.01的pH测量。

To date, numerous studies have been performed to elucidate the complex cellular dynamics in skin diseases, but few have attempted to characterize these cellular events under conditions similar to the native environment. To address this challenge, a three-dimensional (3D) multimodal analysis platform was developed for characterizing in vivo cellular dynamics in skin, which was then utilized to process in vivo wound healing data to demonstrate its applicability. Special attention is focused on in vivo biological parameters that are di±cult to study with ex vivo analysis, including 3D cell tracking and techniques to connect biological information obtained from different imaging modalities. These results here open new possibilities for evaluating 3D cellular dynamics in vivo, and can potentially provide new tools for characterizing the skin microenvironment and pathologies in the future.

Photoacoustic imaging (PAI) is a hybrid imaging method based on photoacoustic (PA) effects, which is able to capture the structure, function, and molecular information of biological tissues with high resolution. To date, therapeutic techniques under the guidance of PAI have provided new strategies for accurate diagnosis and precise treatment of tumors. In particular, conjugated polymer nanoparticles have been extensively inspected for PA-based cancer theranostics largely due to their superior optical properties such as tunable spectrum and large absorption coe±cient and their good biocompatibility, and abundant functional groups. This mini-review mainly focuses on the recent advances toward the development of novel conjugated polymer nanoparticles for PA-based multimodal imaging and cancer photothermal therapy.

Tongue cancer is an increasingly common disease with high morbidity. Besides clinical observation, biomedical imaging techniques have been investigated for early detection of tongue cancer. In this paper, we proposed a co-registered dual-modality photoacoustic (PA) and ultrasound imaging technique to simultaneously map the functional and structural information of human tongue, which has the potential to detect and diagnose tongue cancer in early stage. The imaging probe comprises a 20-MHz side-view focused transducer for ultrasound imaging and PA detection, a light path constructed by a multimode optical fiber, and a prism for PA illumination. Phantom experiments were conducted to evaluate the performance of the system including penetration depth, spatial resolution and signal-to-noise ratio. In vivo imaging of animal tumor and human tongue was carried out to show the feasibility of the proposed technique to detect tumor lesions in human tongue. The results of phantom and in vivo experiments suggest that the proposed technique has the potential to detect the early-stage cancer lesions in human tongue.

Gold nanoparticles (AuNPs) exhibit superior optical and physical properties for more effective treatment of cancer through incorporating both diagnostic and therapeutic functions into one single platform. The ability to passively accumulate on tumor cells provides AuNPs the opportunity to become an attractive contrast agent for X-ray based computed tomography (CT) imaging in vivo. Because of facile surface modification, various size and shape of AuNPs have been extensively functionalized and applied as active nanoprobes and drug carriers for cancer targeted theranostics. Moreover, their capabilities on producing photoacoustic (PA) signals and photothermal effects have been used to image and treat tumor progression, respectively. Herein, we review the developments of AuNPs as cancer diagnostics and chemotherapeutic drug vector, summarizing strategies for tumor targeting and their applications in vitro and in vivo.

为解决卫星灵活机动多模式成像中多片TDI CCD拼接像元失配的问题，提出了一种实现TDI CCD相机成像搭接与错位像元快速拼接配准的匹配算法。通过分析卫星侧摆成像过程焦平面上像移速度矢量变化导致的多片TDI CCD拼接成像像元失配现象，设计了成像速度矢量光线追迹与齐次坐标变换方法，计算了侧摆成像过程中影响图像失配的偏流角、像移速度矢量和两片TDI CCD拼接纵向错位方向和横向搭接方向的像元数目。利用小卫星姿态控制系统半物理仿真平台和TDI CCD原理样机系统对两片TDI CCD拼接像元匹配算法进行实验验证。结果表明：视场角为10°的TDI CCD相机在卫星侧摆30°成像时，经过1个拼接周期后，两片TDI CCD拼接纵向错位和横向搭接像元数目由初始的148.5和50变为137和49，其中横向搭接像元数目与理论计算结果仅有0.1 pixel的偏差，纵向错位像元数目与理论计算结果基本一致。经实验和理论对比分析，所设计的纵向错位与横向搭接像元快速拼接配准算法能够解决TDI CCD 图像拼接的准确性，确保图像像元匹配进而获得高质量的图片。