基于FTIR研究不同年份远志药效物质累积规律

李慧; 李建丽; 苏琪辉; 黄玉荣; 程艳刚; 高蕊蕊; 王颖莉; 詹海仙

doi:doi:10.3964/j.issn.1000-0593(2023)04-1103-09

光谱学与光谱分析, 2023, 43 (4): 1103, 网络出版: 2023-05-03

基于FTIR研究不同年份远志药效物质累积规律

Study on Drug Substance Accumulation of Polygala Tenuifolia in Different Years Based on FTIR

论文大纲

李慧李建丽苏琪辉黄玉荣程艳刚高蕊蕊王颖莉詹海仙

作者单位

山西中医药大学中药与食品工程学院, 山西晋中 030619

远志红外光谱二阶导数双指标序列分析药效物质累积 Polygala Infrared spectrum Second derivative Dual-index sequence analysis Accumulation of active substances

摘要

分析远志不同生长年份特征化学成分的药效物质累积规律。采用傅里叶变换红外光谱法对山西汾阳、新绛12批不同生长年份的远志药材进行红外光谱扫描分析, 建立远志的红外指纹图谱, 并进行共有峰率和变异峰率双指标序列分析。远志药材特征吸收峰在3 130, 2 920, 1 650, 1 540, 1 400, 1 260和1 050 cm-1存在明显的共有峰, 醇提物特征吸收峰位于3 150~3 000, 2 950~2 920, 2 850, 1 740~1 710, 1 670~1 630, 1 540~1 520, 1 450~1 400, 1 100~1 050, 990和530 cm-1范围内；远志吸收峰数目随年限增长呈增加趋势, 同一年份远志药材在3 380, 1 315, 1 060和990 cm-1附近, 醇提物在3 360和1 315 cm-1附近吸收峰的数目、形状和强度存在差异, 秋采远志在此范围内无特征吸收。药效物质累积量: 汾阳产远志、新绛产远志在春季采收药效物质累积量较大；远志有效成分: 前者细叶远志皂苷、 3,6’-二芥子酰基蔗糖、远志口山酮Ⅲ药效物质累积量随远志生长年限的增长累积增加；后者3,6’-二芥子酰基蔗糖的累积量呈先上升后下降的趋势, 远志口山酮Ⅲ和部分皂苷类物质随生长年限的延长呈下降趋势。远志药材共有峰率在66.7%~100%, 变异峰率在0~63.6%, 醇提物共有峰率在66.7%~94.7%, 变异峰率在0~30.0%；同一年限远志样品共有峰率较高, 春采之间、秋采之间共有峰率较高；同一产地远志共有峰率较高, 药效物质累积量随年限增长较为相似, 无明显差异；其中变异峰率最高的药材序列是(春采)S-2-3: S-2-5(秋采)为63.6%, 二者为不同年限不同季节采收, 药效物质累积量相差较大。基于红外光谱法和二阶导数结合双指标序列分析法可以分析探究远志不同生长年份有效化学成分的特征峰变化规律和药效物质累积规律, 为远志的种植采收时间、质量控制和评价提供参考。

Abstract

To analyze the accumulation rule of pharmacodynamic substances of characteristic chemical constituents of Radix polygala in different growth years. FTIR was used to scan and analyze 12 batches of Radix polyphyllum with different growth years in Fenyang and Xinjiang of Shanxi Province. The infrared fingerprint of Radix polyphyllum was established, and the sequence of common peak rate and variation peak rate was analyzed. There were obvious common absorption peaks at 3 130, 2 920, 1 650, 1 540, 1 400, 1 260 and 1 050 cm-1. The characteristic absorption peaks of ethanol extract were in the range of 3 150～3 000, 2 950～2 920, 2 850, 1 740～1 710, 1 670～1 630, 1 540～1 520, 1 450～1 400, 1 100～1 050, 990, 530 cm-1. The number of absorption peaks increased with the increase of age. In the same year, there were differences in the number, shape and strength of absorption peaks near 3 380, 1 315, 1 060 and 990 cm-1 for P. polygonum, and the number, shape and strength of absorption peaks near 3 360 and 1 315 cm-1 for alcohol extract, and there was no characteristic absorption of P. polygonum in autumn. In terms of the accumulation of pharmacodynamic substances, the accumulation of pharmacodynamic substances in Fenyang and Xinjiang polygonum was larger in spring harvesting, and the accumulation of tenuifolin in fenyang polygonum, 3,6’-Disinapoylsucrose, and Polygalaxanthone Ⅲ increased with the growth of polygonum. The accumulation of 3,6’-Disinapoylsucrose increased firstly and then decreased, while the contents of Polygalaxanthone Ⅲ and some tenuifolin decreased with the increase of growth years. The common peak rates were 66.7%~100%, the variation peak rates were 0~63.6%, the common peak rates of alcohol extract were 66.7%~94.7%, and the variation peak rates were 0~30.0%. The common peak rate of the same period was high, and the peak rate between spring and autumn harvests was high. The common peak rate was high, and the accumulation of pharmacodynamic substances was similar to the increase in age, but there was no significant difference. The highest mutation peak rate was S-2-3 (picked in spring) and S-2-5 (picked in autumn), which was 63.6%. The two sequences were harvested in different seasons in different years, and the accumulation of pharmacodynamic substances differed greatly. The characteristic peaks of effective chemical components and the accumulation of pharmacodynamic substances of P. polygonum in different growth years can be analyzed and explored based on infrared spectroscopy and second derivative combined with double index sequence analysis, which can provide a reference for quality evaluation of Polygala tenuifolia.

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李慧, 李建丽, 苏琪辉, 黄玉荣, 程艳刚, 高蕊蕊, 王颖莉, 詹海仙. 基于FTIR研究不同年份远志药效物质累积规律[J]. 光谱学与光谱分析, 2023, 43(4): 1103. LI Hui, LI Jian-li, SU Qi-hui, HUANG Yu-rong, CHENG Yan-gang, GAO Rui-rui, WANG Ying-li, ZHAN Hai-xian. Study on Drug Substance Accumulation of Polygala Tenuifolia in Different Years Based on FTIR[J]. Spectroscopy and Spectral Analysis, 2023, 43(4): 1103.

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