人类表皮生长因子受体-2（HER2）的异常扩增会导致癌细胞的过度增殖和肿瘤恶化。在采用常规光学显微成像技术检测扩增水平较高的乳腺癌细胞HER2基因时，荧光原位杂交探针的荧光信号斑点呈簇状分布，难以精确计数。应用结构光照明超分辨成像技术对HER2基因荧光原位杂交的病理切片进行成像，从而分辨距离较近的荧光探针。通过大视场扫描成像和图像拼接，对数百个细胞进行成像和统计分析，提高了高扩增水平病理切片上HER2探针计数的准确性。

Aberrant amplification of the human epidermal growth factor receptor 2 (HER2) genes can lead to excessive proliferation of cancer cells and tumor progression. When conventional optical microscopy is used to detect the HER2 gene in breast cancer cells with high amplification levels, the fluorescent spots of the fluorescence in situ hybridization (FISH) probe tend to appear as clusters, making accurate counting challenging. In this paper, structured-light illumination super-resolution microscopy is applied to image pathological sections of HER2 gene fluorescence in situ hybridization, in order to resolve fluorescent probes close to each other. Through large field scanning imaging and image stitching, hundreds of cells can be imaged with super-resolution and statistically analyzed. The proposed method allows more accurate enumeration of aggregated fluorescent spots on slides with high-level gene amplification.