激光生物学报, 2019, 28 (4): 314, 网络出版: 2019-09-27  

斑马鱼TAK1正向调控IRF7介导的天然免疫反应

Zebrafish TAK1 Positively Regulates IRF7-mediated Innate Immune Responses
作者单位
省部共建淡水鱼类发育生物学重点实验室, 湖南师范大学生命科学学院, 长沙 410081
摘要
在鱼类中关于转化生长因子β -激活激酶1(TAK1)在天然免疫反应中的功能研究较少。为了探究TAK1在斑马鱼天然免疫中的功能, 本文克隆并获得了一种斑马鱼tak1剪接异构体(Drtak1), 其开放阅读框含有1 737个核苷酸, 编码578个氨基酸, 其中包括N端的丝氨酸/苏氨酸蛋白激酶结构域和C端的卷曲螺旋结构部。通过免疫荧光试验, 证实DrTAK1是一种胞质蛋白。双荧光素酶报告试验显现在EPC细胞中单转DrTAK1不能诱导IFN的产生, 但与IRF7共转时能显著提高其诱导干扰素启动子表达的能力。本文研究结果首次在斑马鱼中发现TAK1能正向调控IRF7介导的天然免疫反应, 为后续DrTAK1功能研究奠定了基础。
Abstract
There are few reports on the function of transforming growth factor β-activated kinase 1(TAK1) in innate immune response in fish. To explore the role of zebrafish TAK1(Danio rerio TAK1, DrTAK1) in innate immune, a new splicing isoforms of Drtak1 has been cloned and characterized in this study. The open reading frame (ORF) of the Drtak1 has 1 737 nt, which can encode 578 amino acids. DrTAK1 includes a N-terminal serine/threonine protein kinases (S/TKc) and a C-terminal coiled-coil region. The results of immunofluorescence showed that DrTAK1 is a cytoplasmic protein. Over-expression of DrTAK1 in EPC cells down-regulates the activity of IFN promoter in luciferase reporter assay in EPC cells. However when co-transferred with zebrafish IRF7, the DrTAK1 can obviously promote the IFN-activation ability of IRF7. The data in this study showed that the DrTAK1 positively regulated IRF7-mediated innate immune responses for the first time in zebrafish, and it laid the foundation for subsequent DrTAK1 research.
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彭俊, 晏俊, 张音音, 冯浩, 肖军. 斑马鱼TAK1正向调控IRF7介导的天然免疫反应[J]. 激光生物学报, 2019, 28(4): 314. PENG Jun, YAN Jun, ZHANG Yinyin, FENG Hao, XIAO Jun. Zebrafish TAK1 Positively Regulates IRF7-mediated Innate Immune Responses[J]. Acta Laser Biology Sinica, 2019, 28(4): 314.

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